| BackgroundMany risk factors contribute to the occurrence and development of ischemic stroke (IS), and all of the factors can be devided into non-genetic factors and genetic factors. Nowadays, there are many studies regarding the relationship and mechanisms between different non-genetic risk factors and IS. However, most of them are limited to the relations of some specific factors to IS. So it is necessary to analyze the non-genetic risk factors of IS systematically to prevent and control IS. At present, many genes and their polymorphisms are associated with IS, and the genetic risk factors of IS have not been conformed exactly. But few studies on the relationship between polymorphisms of adiponectin gene and IS are reported in the world.ObjectiveThe purpose of this study is to explore the risk factors of IS and the relationship between polymorphisms of adiponectin gene and IS in the Han Chinese population, and to prevent and control IS.MethodsA 1:1 matched case-control study method was applied to investigate initial IS cases, The IS cases were selected from two large general hospitals of Shenzhen, and controls were matched to the cases with the same gender and ethnicity, and age differed within 5 years. All the cases and controls were interviewed by the same epidemiology questionnaire. Taqman probe of Fluorescence quantitative PCR was used to detect the genotype of adiponectin gene loci. T test, analysis of variance, trend test, chi-square test, and logistic regression were applied to analyze the data.Results457 initial cases and 457 controls were investigated and the male and female ratio is 1.37:1. There is no statistical difference between the age of cases and controls (P> 0.05), while the average age of cases is 61.75±9.98 years and that is 61.24±10.06 years in controls.Those differential factors in univariate analysis are analyzed further by multivariate logistic regression and the results indicate that high level of LDL(OR=2.85; 95%CI: 1.41,4.23), central obesity(OR=2.20; 95%CI:1.38,3.25), hypertension(OR=7.62; 95%CI:3.26,17.80), family stress(OR=3.13; 95%CI:1.26,7.81), smoking(OR=2.09; 95%CI:1.20,3.62), high level of triglycerides(OR=1.66; 95%CI:1.10,2.49) and high levels of blood glucose(OR=1.24; 95%CI:1.03,1.49) were significant independent risk factors for IS. Whereas, the independent protective factors of IS were physical activity(OR=0.11; 95%CI:0.02,0.51), partial retirement or professional activity (OR=0.28; 95%CI:0.11,0.73), afternoon napping(OR=0.30; 95%CI:0.13,0.71), high level of HDL(OR=0.38; 95%CI:0.15,0.93), tea drinking(OR=0.41; 95%CI:0.18,0.94), consumption of vegetable oil in two recent years(OR=0.46; 95%CI: 0.31,0.69), high-frequency consumption of other vegetables(OR=0.60; 95%CI:0.44,0.83), and high level of total protein(OR=0.66; 95%CI:0.50,0.88).C>G mutation of rs266729 of Adiponectin gene decreased the risk of IS in the Han Chinese population(OR=0.71; 95%CI: 0.55,0.93); and T>G homozygous mutation of rs2241766 was related to the susceptibility of Han Chinese women (P=0.04). However, C>G mutation of rs266729 were not statistically significant after the adjustment of the differential varables in Univariate analysis (P=0.40).Conclusionscentral obesity, high level of LDL, triglycerides, and blood glucose, hypertension and smoking remain to be the main risk factors of IS in Han Chinese population. Some noval risk factors have been emerging and changing the susceptibility of IS, such as family stress, physical exercise, and physical activity. The C>G mutation of rs266729 decreases the risk of IS, but it is not the independent protective factor of IS. |
PDF Full Text Request