Studies On The Anxiolytic Effect And Function Mechanism Of Ganmaidazao Tang

Objective: Studies on the anxiolytic effect and function mechanism of Ganmaidazao Tang.Methods: Take the mice as the object of study, application EPM duplication anxious animal model, from behavior study angle the anti-anxious function. From behavior study angle factors and so on observation sex, weighe, testing time to EPM experiment result influence; Based on this research about dose-response relationship and time-concentration of the Ganmaidazao tang anti-anxious; Used HPLC-EC technique, in determination the region of hippcampus mice 5-HT content change, determined Ganmaidazao Tang affects anxious function mechanism.Results:1. Sex to experimental result influence:female group and male group OE%,OT% and locomotor activity P>0.05.2. Weight to experimental result influence: compares with the weight for 20.0±1.0g group, the weight for 15.0g±1.0g group OE% P<0.05, 25.0g±1.0g group OE%P<0.01;15.0g±1.0g and 25.0g±1.0g group OT%P<0.01; 15.0g±1.0g group locomotor activity P<0.05.3. Test time to experimental result influence:compares with the 14:00-17:00 group, 19:00-21:00 group OE% P<0.05; 9:00-12:00 group OT% P<0.05, 19:00-21:00 group OT% P<0.01; 9:00-12:00 group locomotor activity P<0.01.4. The experimental results of the dose-response relationship showed that from the doses of 7.0g/kg started work there was significant difference OE% P<0.01; the doses of 14.g/kg reached the climax, there was extremely significant difference between the OE% and OT%; the doses of 3.5g/kg OT% P<0.05.5 . The experimental results of the time-response relationship showed: the experimental results of the time- concentration relationship showed that giving medicine for three days started work, OE% P<0.05; giving medicine for seven days reach the climax, there was extremely significant different between OE% and OT% P<0.01; giving medicine for five days OT% P<0.05.6. The experimental results of function mechanism of Ganmaidazao Tang, it can reduce content 5-HT of the region of hippocampus mice P<0.01.Conclusion:1. The sex hasn't affect EPM results.2. The weight affects EPM results: weight of 19.0g-21.0g is better.3. Test time affects EPM results: test time of 14:00-17:00 is better.4. Ganmaidazao Tang could improve the OE% and OT%,14.0g/kg is choosed for the best doses, seven days is choosed for the best time.5. Ganmaidazao Tang can reduce content 5-HT of the region of hippcampus mice...