| Suanzaoren Tang(SZRT) is derived from "Synopsis of Prescriptions of the Golden Chamber", the indications is agitation of deficiency type result from insufficient liver blood, deficiency-fire stirring up inside. In modern clinic practice, it can treat many psychological disorders including anxiety as well as insomnia. On the basis of proving that SZRT decoction can treat anxiety, the relationship between different component compatibilities and their effects of treating anxiety was reviewed through isolating and recombining the main components in the decoction, meanwhile, the anxiolytic effect of effective components compatibilities was discussed elementarily .This paper includes summarization and experiment study two parts. The summarization includes study progress about compound prescriptions of traditional Chinese medicine and study status in quo of clinic, pharmacology and chemistry about SZRT. The experiment includes isolation of components, drug effect observation of component compatibilities, action mechanism of effective components.Experiment1: 6 component groups were isolated from SZRT decoction by means of system isolation, and the nature determination and obtain percent were made. The obtain percent is as follows:A(volatile oil),B(polysaccharide,11.49%), C(diethyl ether, sapogenin and flavone, 0.15%), D(saponin and sapogenin, 13.31%), E(flavone, 0.22%),F(the rest part, 0.065%).Experiment2:6 components were formulated according to orthogonal meter and got SZRT1-SZRT8. Kunming mice were administrated orally by herb medicine(20/kg/day) and Wister rats were administrated orally by herb medicine (7.5/kg/day), it's 2mg/kg/day and 1mg/kg/day to Diazepam group, the elevated plus-muse(EPM) test was performed after 10 days of administration.The results were: percentage of open arm entries (OE%) and of times spent in the open arms (OT%) of SZRT1(A＋B＋C＋D＋E＋F)and SZRT6(B+E)mice were both higher than that of control group(p<0.05). In the mouse EPM experiment, OE% of SZRT3 and SZRT5 groups were higher than that of control group(P<0.05), but there was no significant difference between every group and control group about total number entries (P>0.05). In therat EPM rats were higher than that of control group(P<0.05),but the total arm enteries had no significant difference compared with that of the control group(P>0.05). In two experiments, the effects of SZRT1 and SZRT6 were similar to Diazepam; there was no significant difference about the index between SZRT6 and SZRT1 group. The results showed: SZRT6 and SZRT1 had the effects of anti-anxiety, and it was not related to sedation and hypnosis effects; SZRT6(B+E) had close relationship with anti-anxiety effect besides the recipe including all components. The effects of all different components of SZRT on OE%,OT% showed components C only effected OE% significantly(minus),components E only effected OT% significantly(positive). Components B and E had cooperation effect in the respect of anti-anxiety.Experiment3: Central neuropeptide, monoamine transmitter and serum cytokine were measured by means of high performance liquid chromatography coupled with electrochemical detection (HPLC-ECD) and radioimmunoassay. The test of neuropeptide showed that the β-endorphin in SZRT1 and SZRT6 group mice increased significantly(P<0.05); Neuropeptide Y concentration in SZRT6 group rat hypothalamus decreased significantly(P<0.05), so did that in SZRT1. Neuropeptide Y in Nucleus amygdalae increased significantly(P<0.05), the same to diazepam group. The results showed SZRT1 and SZRT6 might accelerate the excretion of β-endorphin in hypothalamus and accelerate transport neuropeptide Y in hypothalamus to Nucleus amygdalae.The test of monoamine transmitter showed that 5-HT concentration in SZRT6 group rats' hippocampus decreased significantly(P<0.05), SZRT1 group decreased without significant, the effects were similar to diazepam group, SZRT1 and SZRT6 might decrease the releasing of 5-HT in rat hippocampus. The test of cytokine showed that SZRT1 and SZRT6 cou... |
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