Study Of Phthalate Metabolites In Urine Of Pregnant Women

Objective:Testicular dysgenesis syndrome is a cluster of diseases, including cryptorchidism, hypospadias, testicular cancer, reduced sperm production. Some reseaches have suggested that there's a strong connection between Testicular Dysgenesis Syndrome and Endocrine disrupting chemicals, especially phthalate metabolites. our reseach try to exhibit the exposure of phthalate metabolites in pregnant women via determing and analysing three phthalate monoesters in urine sample and investing the women's age, life style,. To provide data for the future reseach of TDS mechanism.Method:A solid-phase extraction-liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS) method was developed for the analysis three phthalate monoesters including Monomethy phthalate (MMP), Monobutyl phthalate (MBP), Monoethyl phthalate (MMP) in urine samples of 100 pregnant women, Zhejiang province. Pearson X2 was used to assess the relation between three phthalate monoesters and influential factors including age, plastic products, cosmetics and 5-year house decoration via SPSS 17.0. A p-value of<0.05 indicated statistical significance.Results:Phthalate monoesters were found in 88% of the samples, MBP has the highest rate of 72%, with 41% of MMP and 21% of MEP. there are no relations found between Phthalate monoesters and 5-year house decoration, age. the rates of MBP and MMP are higher in the urine samples of pregnant women who frequently use the plastic products (p<0.05), the rate of MBP is higher in the urine samples of pregnant women who frequently wear perfume and other cosmetics(p<0.05)。 Conclusion:The exposure of phthalate metabolites is common in pregnant women, Zhejiang province. especially in the women who use plastic products and cosmetics in daily life.