| YiQiYangYin Fang(益气养阴方),Be composed of American ginseng, fresh rehmannia root, Fragrant Solomonseal Rhizome, all-grass of snake foot clubmoss etc, has effects of supplementing qi and nourishing yin, promoting the production of body fluids and increasing exercise capacity. YiQiBuShen Fang(益气补肾方),Be composed of ginseng, Lucid Ganoderma, polygonatum sibiricum Delar etc, has effects of supplementing qi and tonifying kidney, nourishing body, delaying senescence and getting rid of illnesses. In addition to the common effect of supplementing qi, YiQiYangYin Fang has unique clinical application in exercise-induced fatigue disease because of its function of reinforcing yin. YiQiBuShen Fang is used for treating senescent diseases by reason of its function of invigorating kidney.Objective:the present study, using modern pharmacological techniques under the guideline of TCM theories, studied the immunoregulation and anti-stress, anti-hypoxia effects of YiQiYangYin Fang and YiQiBuShen Fang, and summarized the progress in the treatment of exercise-intrigued fatigue and senescence.We also investigated the sports fatigue antagonizing effect of YiQiYangYin Fang and anti-aging functions of YiQiBuShen Fang.Methods:The immunological studies were carried in mice immunosuppressed by cyclophosphamide. The anti-stress and anti-hypoxia effects were evaluated in mice bearing hypoxia at normal pressure. Sport fatigue model and natural aging model were utilized to investigate the anti-fatigue of YiQiYangYi Fang.and anti-aging mechanisms of YiQiBuShen Fang.Results:1 Both prescription can obviously increase the thymus index and of the mice immunosuppressed by cyclophosphamide, cunter the loss of weight of immunological organs caused by immunosuppressor such as cyclophosphamide, and thus enhance immunological activies.2 Both prescription possess anti-stress and anti-hypoxia properties. Gasp time of decapattive mice prolongation were observed in subgroups of 14.28g/kg and 7.14g/kg respectively, which is indicative of protcctive effect against cerebral ischemia. Subgroups of all doses showed a trend of lengthening the survival time of mice bearing hypoxia at normal pressure.3 The anti-fatigue mechanisms of YiQiYangYin Fang3.1 Effect against spleen indexa and thymus index showed an increasing trend with YiQiYangYinFan groups compared to the model group.3.2 Compared with the model group, YiQiYangYin Fang at 6.68 g/kg doses could significantly improve the content of Hb of the mice. It is suggested that the anti-fatigue effects of YiQiYangYin Fang was related to the improvement of Hb content.3.3 Compared with the model group, YiQiYangYin Fang at13.36,6.68 and 3.34g /kg doses could dramatically reduce BUN and increase LDH, but had the trend of decreasing LD,CK, NH3 of the mice. It is suggested that the anti-fatigue mechanism of YiQiYangYin Fang was related to reduce BUN and increase LDH.3.4 Compared with the model group, YiQiYangYin Fang atl3.36,6.68 and 3.34g /kg doses could remarkably decrease C, but have a trend of increasing T of the mice, the most significant effect was observed at 13.36g/kg dose level. It is suggested that the anti-fatigue mechanism of YiQiYangYin Fang was related to decrease C.3.5 Compared with the model group, YiQiYangYin Fang at13.36g/kg dose could dramatically increase the muscle and hepatic glycogen. It is suggested that the anti-fatigue mechanism of YiQiYangYin Fang was related to improve energy metabolism.3.6 Compared with the model group, YiQiYangYin Fang can obviously decrease the inflammatory cell infiltration of unilateral ureteral and stromal desmoplasia in kidney. There are no significant changes in testis on all the groups.4 The anti-aging mechanism of YiQiBuShen Fang4.1 Subgroups of all doses showed a trend of increasing the thymus index and spleen index of the mice.4.2 Compared with the model group, YiQiBuShen Fang had no effect on blood cell (WBC,RBC,Hb,PLT)4.3 Compared with the model group, YiQiBuShen Fang at 11.42 and5.71g/kg doses could significantly decrease TG.with no significant effect on GLU and TC, YiQiBuShen Fang Fang have the trend of increasing HDL-C and decreasing LDL-C,. It is suggested that the anti-aging mechanism of YiQiBuShen Fang was related to decrease TG.4.4 Compared with the model group, YiQiBuShen Fang Fang at 11.42 and 5.71g /kg doses could dramatically increase SOD while decreasing MDA,MAO and hepatic,brain lipofuscin. It is suggested that the anti-aging mechanism of YiQiBuShen Fang was related to increase SOD and decrease MDA,MAO and hepatic,brain lipofuscin.4.5 Compared with the model group, YiQiBuShen Fang had a T increasing trend.4.6 Compared with the model group, YiQiBuShen Fang can obviously decrease the deficiency of tubules seminiferous spermatogenic cell and stromal desmoplasia in testis.Conclusions:The result showed that YiQiYangYin Fang and YiQiBuShen Fang have immunoregulation and anti-stress, anti-hypoxia properties. Furthermore, YiQiYangYin Fang has the effect of anti-fatigue, the mechanism of which might be related to the increase of Hb, T, LDH and muscle and hepatic glycogen, and the decrease of BUN, C. while YiQiBuShen Fang has the effect of anti-ageing, the mechanism of which might be related to increase the content of SOD, and decrease the content of TG, MDA, MAO, and the hepatic and brain lipofuscin. All these results provided pharmacology basis for their efficiency and clinical application.
|
PDF Full Text Request