| Method: Take the HER2 stomach cancer cells logarithmic growth after 0.25% of trypsin digestion sterile PBS rinse cells with twice, centrifugal, serum-free medium heavy DMEM/join the suspension cells, adjust its concentration of seven by 7×10~6/ml, each Balb/c mice inoculation only 200μlcells in its back, suspension liquid. Only 12 cells to seed HER2 Balb/c mice gastric cancer cell transplantation will be HER2 randomly divided into two groups and each group only 6: the control group treated with, his daughter. Treatment for his daughter: 10 mg dose; bw/kg Usually give medicine again. Handled 3 w, every 3 d with caliper measurement transplantation and calculation of implant tumors secreting change the size melanoma. Animals out rapidly after mass death with saline wash, after weighing and filter paper blot moisture in darfur with 10% neutral formalin fixed, dehydration, merlin, conventional paraffin embedding biopsies, used to make pathological and immunohistochemical.Results: Compared with controls, can significantly reduce daughter function group mice gastric cancer cells high HER2 express the volume and effective transplant tumor change its histopathological structure. Ki - 67 antigen IHC experimental results show that the control group transplant tumor cells more, its positive particles within reach 56.2% positive. After by his daughter, its positive handle 3w and dyeing strength declined obviously, and its positive rate reached 43.2% (P < 0.01).Conclusion: Gastric cancer cells express high HER2 reduced volume and the transplant tumor effectively change its histopathological structure in the body, can effectively restrain the daughter of tumor cells of Balb/c mice transplantation HER2 periodic process.
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