Correlation Study Of The TNF-α And Psoriasis

In order to overall elucidate the relationship between TNF- a and psoriasis, we designed this experiment.1. We collected sera and lesional skins from 33 patients with typical psoriasis vulgaris, also from 22 healthy volunteers. In the same time, we detailly record the course of the disease, the age, the treatment and the psoriasis area and severity index (PASI) score of the 33 patients.2. We carried out ELISA for TNF- in sera of patients and healthy volunteers, then used Stata software to analyze the relationship between TNF- levels and the course of disease, the age and the PASI score. Also we compared the TNF- levels of patients with the levels of volunteers.3. We using immunohistochemical localization by anti- TNF- monoclonal antibody (Z12 antibody) and anti-p55 TNF-R antibody to show the distribution of TNF- and p55 TNF-R in lesions of psoriatic patients, then to compare the different expression between the patients and healthy persons.4. We used L929 cells to detect the cytotoxicity of sera of psoriatic patients and of healthy volunteers, then to compare the cytotoxicity between the patients' and the volunteers' . Also we used Stata software to analyze the relationship between the cytotoxicity and the course of disease, the age and the PASI score in patients. Then we used anti-TNF- monoclonal antibody (Z12 antibody) to neutralize the sera of patients, after that used ELISA and L929 cells to detect the TNF-a concentration and bioactivities in neutralizing sera.To sum up, we can find that TNF-α is closely related with psoriasis. TNF- a through combination with p55 TNF-R to exert its biological properties. Therefore, we will use drugs to neutralize TNF-α or to block the combination with TNF-α and its receptor, so that to lessen patients' manifestations. That is an important development in the biological treatment of psoriasis in the future.