Quality Control And Fingerprint Chromatograph Research By HPLC Of Rhodiola Crenulata (Hook.f.et Thoms) H. Ohba.

Objective:To establish simple and effective method for rapid identification and fingerprint of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba. Providing a system scientific method for scientific evaluation and effective control of the quality of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba.Methods:Maroscopic,microscopic and TLC traditional identification methods,combining HPLC method were performed to distinguish and compare the quality of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba;To build the fingerprint of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba using the HPLC method of gradient elution, and evaluate the similarity effectively.Result:1.We can obiviously identify Rhodiola crenulata (Hook.f.et Thoms)H. Ohba and its counterfeits from the shape,surface color.And the remnants of yellow rhytidome on Rhodiola crenulata (Hook.f.et Thoms)H. Ohba is another very important feature of distinguish it and its counterfeits.2.The rhytidome is easy to flake off, the shape of the nomal collateral bundle was long and slender, its phloem is shorter than xylem, there are many phellem bands,the abnormal vascular bundles exist in the medulla lonely or as compound types composed by two or three abnormal vascular bundles,and there are also several transverse vessels.Ale of these are important microscopic features can be used for identification of Rhodiola crenulata (Hook.f.et Thoms)H.Ohba.3.The dot was not found in the Rhodiola fastigiata(Hk.f. et Thoms.)S. H.Fu and Rhodiola quadrifida (Pall.)Fisch.et Mey. Enum in the corresponding location with salidroside by TLC.This study supplies a. reliable and simple reference for identification of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba.4.The results of the determination of salidroside showed that in 17 batches of Rhodiola crenulata(Hook.f.et Thoms)H.Ohba,the number of the content of salidroside which meet China pharmacopoeia was 16. (Based on dry goods, the content of salidroside should not be less than 0.5%.)The content of salidroside varies with grades of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba. The higher the grade,the higher the content. But the salidroside was not be found in Rhodiola fastigiata(Hk.f.et Thoms.)S.H.Fu and Rhodiola quadrifida (Pall.)Fisch. et Mey.5.Established the chromatographic fingerprint of Rhodiola crenulata (Hook.f.et Thoms)H.Ohba.11 peaks were identified as the characteristic fingerprints of Rhodiola crenulata.The similarity of Rhodiola crenulata was calculated.And the components of Rhodiola crenulata and its counterfeits are obviously different.Conclusions:1.The traditional methods of Maroscopic,microscopic and TLC identification were simple and reliable, and can be used for initial identifying Rhodiola crenulata (Hook.f.et Thoms)H. Ohba. and its counterfeits.2.Using HPLC method for determination of salidroside in Rhodiola crenulata and its confused species would furth identify them.Besides, this method can provide scientific information for quality control of Rhodiola crenulata (Hook.f.et Thoms)H. Ohba effectively. This is a simple and accurate reproductive method which can be used for determination of salidroside of Rhodiola crenulata and its confused species.3.The establishment of fingerprint of Rhodiola crenulata (Hook.f.et Thoms)H.Ohba by HPLC and evaluating its similarity showed that this method can control the quality of Rhodiola crenulata simply and scientifically, and also provide a more scientifically and directly performance for identifying Rhodiola crenulata and its confused species.