The Experimental Study Of Establishmen Rat Model Of Bile Duct Benign Stricture

ObjectiveExplore a simple,economical,practical stable and reliable rat model of bile duct benign stricture, to research the pathogenesis and effective treatment methods of bile duct benign stricture.Methods49female SD rats, weight 220-260 g, average 240±10 g, randomly divided into the experimental group 21,the control group 21 and the blank control group7,all the experimental animals will be injected with the 3% of pentobarbital for celiac anesthesia. the experimental group was laparotomy under sword axon in epigastric abdominal next midline incision after anesthesia,exposue the common bile duct and separation it on 1 cm in the distance from Duodenum, placed a gauze thick about 0.2cm behind the common bile duct, dripped about 0.7ml of the 95% alcohol on it to continue 2-3minutes and then closed abdominal; The control group was surgeryed as the same method except for dripping the Physiological saline of 0.9%. The blank control group only search for the bile duct without doing other thing.We must observe the mental state,urine color,eating situation after the surgery and so on. Selected 5 tested animal to weigh and draw blood to test the liver function(include ALT,GGT,T-BIL,D-BIL),and cut a part liver and the tested bile duct for pathology examination after the postopration 7,14,21 day, in order to understand the situation of bile duct obstruction through biliary imaging after the postopration 21 day. Using SPSS software, t -test and multivariate analysis of variance.ResultsSD rats turn over in 1hour, water after 3-4h, gradual return food.①the experimental group test the ALT,GGT,T-BIL,D-BIL etc.There is a obvious change(P<0.05)from preoperation to the 21 day of postoperation;But the control group no obvious change.②Killed the rat in the right time to cut the liver and bile duct for pathology examination, to find liver cell vary degrees injury and the part of the bile duct organization fiber hyperplasia in the experiment group,however there is no variation on the control group from preopration to postopration.③There is a obvious bile duct obstruction through biliary imaging after the 21 day of postopration.but the control group no bile duct obstruction. Through the comparison, the experimental group and the control group was statistically significant (P<0.05), the control group and the blank control group was not statistically significant (P> 0.05).ConclusionsIt is viable to drip the 95% alcohol on the outside of liver bile duct to build the bile duct benign stricture on rat in this experiment,it can offer another good animal model for research the bile duct benign stricture.