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Screen The Anti-tumor Active Part And Preliminarily Research The Chemical Constituents Of The Tubers From Typhonium Giganteum Engl

Posted on:2012-11-20Degree:MasterType:Thesis
Country:ChinaCandidate:C C WangFull Text:PDF
GTID:2154330335473217Subject:Medicinal chemistry
Abstract/Summary:PDF Full Text Request
In order to explore the antitumor active components of Typhonium giganteum Engl. and make it reasonably developed and utilized. The powder of Typhonium giganteum Engl. tuber was extracted by supercritical fluid CO2 extraction and homogenate extraction, ether layer and methanol layer were obtained from the supercritical extract by using ether as extraction solvent, dichloromethane layer, n-butanol layer and water layer were acquired from the homogenate extract by using three different extract solvents (dichloromethane, n-butanol and water) in order. The quantification and the identification of the two low-polarity fragments were accomplished using Gas chromatography-mass spectrometry(GC-MS) technology. MTT colorimetric method in vitro was used to explore the inhibiting effect of five extract on the proliferation of eight human cancer cell lines, and the cytotoxicity was evaluated using IC50 value. The author isolated and identified two compound (β-Sitosterol and Daucosterol) from the dichloromethane layer. The in vitro antitumor effect ofβ-Sitosterol on five human cancer cell lines was evaluated by MTT assay. This study provided scientific basis for delve into the antitumor active components of T. giganteum Engl. and reasonably and efficiently develop and utilize T. giganteum Engl..1. Compared the supercritical CO2 extraction and homogenate extraction, we can know:The extraction ratio of ether layer obtained from the supercritical CO2 extract of T. giganteum Engl. was slightly higher to dichloromethane layer acquired from homogenate extract of T. giganteum Engl., but speed of homogenate extraction was faster. Compared to supercritical CO2 extraction, homogenate extraction was more suitable for large-scale production.2. The two low-polarity fragments were identified and compared by GC-MS analysis, we can know:The composition of the ether layer and dichloromethane layer were different, the content of the same component were also different. But the major components of the two extract were almost consistent, Three substance thatβ-sitosterol, palmitic acid and linoleic acid all were the main ingredient of the two extract and respectively accounted for 57.89% in ether layer and 64.77% in dichloromethane layer.3. By compared in vitro inhibiting effect of five fragments on eight human cancer cell lines evaluated by MTT colorimetric method, we can know:Among the five fragments, only the ether layer and the dichloromethane layer had antitumor activity, but the two extract had inhibiting effects only on five human cancer cell lines that gastric cancer SGC-7901, ovarian cancer HO-8910, colon cancer HCT-116, liver cancer BEL-7402 and liver cancer SMMC-7721.Different human cancer cell lines had different sensitivity for the two extract. In the eight human cancer cell lines tested, liver cancer SMMC-7721 cells were most sensitive to the two extract. The results not only confirmed the tuber of T. giganteum Engl. had antitumor effect, but also indicated the antitumor active components concentrated in the low-polarity fragments.4. Two components identified in dichloromethane layer wereβ-sitosterol and daucosterol. The in vitro antitumor effect ofβ-sitosterol on five human cancer cell lines was also evaluated by MTT assay. The result not only showedβ-Sitosterol had antitumor activity and liver cancer BEL-7402 cells were most sensitive to it, but also demonstrated P-sitosterol was the major antitumor active compound in the tuber of T. giganteum Engl..
Keywords/Search Tags:Typhonium giganteum Engl., Supercritical fluid CO2 extraction, Homogenate extraction, GC-MS analysis, MTT colorimetric method, Anticancer activity
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