The Expression Of The Tumorsuppressing Gene Maspin And ER In The Endometriosis

Objectice: Endometriosis(EMs) is one of common gynecological disorders, affecting 10%-15% of women during their reproductive age.At present,it still isn't clear about the endometriosis pathogenesis.Although it is benign pathological changes,it has aggressive and evaluative behavior of malignant tumor.Moreover,EMs is a estrogen-dependent disease.Through investigate the expression and relevance of the tumor suppressor gene Maspin and Estrogen Receptor(ER) in ectopic endometrium and eutopic endometrium of patient with endometriosis(EMs) and in eutopic endometrium of patients without EMs to exploring the role of and in the pathogenesis of endometriosis.Method:All the samples were obtained from the First Hospital of Qinhuangdao City during September 2008 to September 2010. The ectopic endometriotic endometria were obtained from 40 patients with endometriosis(proliferatice phase:n=20;secretory phase:n=20). The patients were undergoing laparotomy or laparoscopy.26 patients with ovarian endometriosia,11patients with peritoneal endometriosia,3 patients with uterosacral ligament endometriosia. The eutopic endometrium of the EMs patients were sampled by hysterectomy or curettage(proliferatice phase:n=20; secretory phase:n=20).In the same time,the control endometrial specimens were obtained from 40 patients who wasn't diagnosed hysteromyoma (proliferatice phase:n=20;secretory phase:n=20). No difference in age was found in research group and compare group (P>0.05).None of the patients received any hormonal therapy during the 6 months before their operation.All the tissues were fixed in neutral-buffered 10% formalin solution.Then 4μm thickness paraffin-embedded sections were cut.The expression of Maspin and ER was detected by the SP immunohistochemically.The experimental dates were obtained by SPSS13.0 for windows.Results: 1 The positice rates of Maspin in ectopic endometrium, eutopic endometrium and control group,were 42.5%,50%,70%,respectively.The expression of Maspin in ectopic endometrium of EMs is lower than eutopic endometrium(P<0.05); the expression of Maspin in ectopic endometrium of EMs is lower than control endometrium(P<0.05); the expression of Maspin in eutopic endometrium of EMs is lower than control endometrium(P<0.05).2 There was no significant differences of the Maspin's expression between proliferative phase and secretory phase in ectopic endometrium with EMs(P>0.05); In the eutopic endometrium with EMs and the control endometrium group, the expression of Maspin in secretory endometrium is higher than that in proliferative (both P<0.05).3 The positice rates of ER in ectopic endometrium, eutopic endometrium and control group,were 62.5%,75%,55%,respectively.The expression of ER in ectopic endometrium of EMs is lower than eutopic endometrium(P<0.05); but there is no statistical difference between ectopic endometrium of EMs and control endometrium(P>0.05);the expression of ER in eutopic endometrium of EMs is higher than control endometrium(P<0.05).4 There was no significant differences of the ER's expression between proliferative phase and secretory phase both in ectopic endometrium and the eutopic endometrium with EMs(P>0.05); Expression of ER in proliferative endometrium is higher than that in secretory in contrlo endometrium (P<0.05).5 The relationship of Maspin and ER:In the secretory phase of ectopic endometrium with EMs,the expression of Maspin was negatively correlated with ER expression (P<0.05). However,in the proliferative phase of ectopic endometrium with EMs, there was no significant correlation between expression of Maspin and ER(P>0.05).In the both secretory and proliferative phase of eutopic endometrium with EMs, there was no significant correlation between expression of Maspin and ER(P>0.05). In the both secretory and proliferative phase of control endometrium,the expression of Maspin was negatively correlated with ER expression (P<0.05). Conclusions:1 The expression absence of Maspin is possibly induce the biological behaviour of EMs similar to malignant tumor.2 Estrogen receptors may be responsible for the occurrence and development of EMs.3 The upset balance between Maspin and ER maybe responsible to the pathogenesis of EMs.