The Expression And Clinical Significance Of P63,Maspin And Caspase-3 In Human Laryngeal Squamous Cell Carcinoma

Objective:To investigate the expression of p63,maspin and caspase-3 in laryngeal squamous cell carcinoma(LSCC)and it's relationship with clnicopathological features,such as cell differentiation,staging,diseased region and metastasis.To explore their roles in tumorigenesis and progression of laryngeal cancinoma.To study the correlation of p63,maspin and caspase-3 in LSCC.To discuse the modulation of p63 and maspin in LSCC.Materials and methods:Formalin-fixed and paraffin-embedded tissues from 30 cases laryngeal squamous cell carcinoma(LSCC),20 cases atypical hyperplasia of vocal cord and 20 cases vocal cord polyps were evaluated for the expression of p63,maspin and caspase-3 by immunohistochemistry.Freash tissues from 20 cases laryngeal carcinoma,20 cases adjacent nontumorous laryngeal epithelium and 10 normal laryngeal epithelium were examined the expression of TAp63 and△Np63 by RT-PCR.Results:immunohistochemistry:The expression of p63 was identified in 25 out of 30 cases LSCC(83.3%),11 out of 20 cases atypical hyperplasia of vocal cord(55%)and 6 out of 20 cases vocal cord polyps(30%).There was significant difference among them(p＜0.05).No statistically significant correlations were found between p63 protein expression and the clinicopathological characteristics of tumor site,clinical stage,pathological grading and lymph node metastasis(p＞0.05).The expression of maspin protein was found in 23.3%of LSCC,35%of atypical hyperplasia of vocal cord and 40%of vocal cord polyps,there was no significant difference among them(p＞0.05).Significant correlation was found between maspin expressin and lymph node metastasis in laryngeal carcinoma(p＜0.05).The results show that the expression of maspin in lymph node metastasis group was significant lower than that of non-lymph node metastasis group in LSCC.In 30 cases LSCC,20 cases atypical hyperplasia of vocal cord and 20 cases vocal cord polyps,the positive rates of caspase-3 were 46.7%,60%,85%,respectively.there was significant difference among them(p＜0.05).The expression of caspase-3 was related to clinical stage and lymph node metastasis(p＜0.05),but not related to tumor site and pathological grade(p＞0.05).In LSCC,there was positive significant correlations between maspin and caspase-3,but there was negative significant correlation between p63 and caspase-3(p＜0.05).RT-PCR:TAp63 was expressed in normal laryngeal epithelium but down-regulated in adjacent nontumorous laryngeal epithelium and LSCC(p＜0.05).The positive rate of△Np63 was 30%in normal laryngeal epithelium,70%in peri-carcinoma tissues and 100%in LSCC.Moreover,the expression of△Np63 mRNA in laryngeal carcinoma was significant higher than that in adjacent nontumorous laryngeal epithelium and normal laryngeal epithelium(p＜0.05).In LSCC,the expression of TAp63 mRNA was related to clinical stage(p＜0.05),but not related to tumor site,pathological grade and lymph node metastasis(p＞0.05).The expression of△Np63 mRNA was related to clinical stage and pathological grade,but not related to tumor site and lymph node metastasis.Conclusion:1.There was over-expression of p63 protein in laryngeal carcinoma. up-regulated expression of p63 may play an regulatory role in the laryngeal carcinoma. 2.Down-regulated expression of TAp63 and up-regulated expression of△Np63 play an important role in the tumorgenesis and development of LSCC.3.As a tumor suppressor gene, maspin may have inhibitory effects on invasion and metastasis of LSCC.4.caspase-3 may be used to evaluate the biological behavior of LSCC in clinic.5.over-expression of P63 can reduce expression of caspase-3 to make tumor cell apoptosis disorder.Up-regulated expression of maspin can suppress the development of LSCC by inducing tumor cell apoptosis.