| Objective: By varying detect and analysis the concentration of cathepsin K and IL-17 in gingival crevicular fluid with chronic periodontitis, to discuss not only the alteration but the meaning of cathepsin K and IL-17 occurred in patients with the evolve chronic periodontitis, and provide clinical diagnosis and treatment reference of chronic periodontal disease.Methods: Selected 87 patients who were diagnosed with chronic periodontitis in January 2010-December 2010 at the Second Hospital of Hebei Medical University. 27 cases of periodontal healthy volunteers. Informed consent was obtained from all participants. All participants without systemic disease, no history of antimicrobial or anti-in?ammatory (aspirin, ibuprofen, etc); no history of therapy or periodontal treatment for 6 months; non-pregnant women; no smokers.To group the object according to gingival index (GI), probing depth (PD), attachment loss (AL), X-ray showed alveolar bone level (ABL).Control group(A): The healthy population, probing depth≤3mm, no attachment loss and alveolar bone absorption. Mild(B): probing depth≤4 mm, attachment loss 1 ~ 2 mm, alveolar bone resorption of root length less than 1/3. Moderate(C): probing depth≤6 mm, 3 ~ 4 mm attachment loss, absorption of alveolar bone more than 1/3 of the root length, but less than 1/2. severe(D): probing depth> 6 mm, attachment loss≥5 mm, alveolar bone loss more than 1/2 of the root length.All research subjects seleceded a maxillary molar, and no caries and pulp, periapical lesions, without occlusal trauma, no acute gingival inflammation. Records for each of the indicators tested GI, PD, AL, ABL.Cut 5 mm of 30 blue Moisture absorption paper point, then ultraviolet irradiation two hours, Operate in the sterile console. Four moisture absorption paper point were divided into one group. And were placed in 0.5ml sterile Eppendorf (EP) tube. Number and electronic weighing scales, records aside.To wear sterile gloves before clinical operation, first of all to remove parts of the subgingival plaque, calculus carefully. The area was isolated by using cotton rolls to avoid saliva contamination. Dried the tooth 1min, gently and insert the tooth mesialbuccal, distalbuccal, mesiallingual, distallingual four sites slightly, stop when come across of minor resistance basis, remove after 30S (pollution by blood is abandoned). Put four Moisture absorption paper point into the original EP and weighed. Record net weight of the original, that is the weight measured by GCF. EP tube recorded after sealing, -80℃cryopreservation standby. All the process were finished independently.Samples thawed at room temperature until completely thawed, add 150μl PBS (PH=7.4,0.01mol/L), micro-mixer shock 2min, 5min intervals and then shock 2min, 4℃, 3000r/min centrifugal 10min. Use Micro pipette to divid 100μl supernatant into two EP tube 50μl each one, send cathepsin K and IL17 to bio-company testing. Detected cathepsin K and IL-17 by the method of ELISA.Analysis the different groups of cathepsin K and IL-17 concentration by using SPSS13.0 statistical software, and comparisons between groups with SNK-q test, the standard significance a=0.05, P<0.05 as statistically significant to analysis.Results:1 The mean concentrations of Cathepsin K:The mean of group A is 52.27±5.09pg/ml, the mean of group B is 68.07±3.82pg/ml, the mean of group C is 64.34±7.14pg/ml, the mean of group D is 60.14±5.54pg/ml. Cathepsin K concentrations of experimental group are higher than those incontrol group (group A) (P <0.05). The concentrations of cathepsin K are decrease with the severity increasing of chronic periodontitis, and the four groups are significant (P<0.05). The concentrations of Cathepsin K is negative correlation with PD, AL, ABL . 2 The mean concentrations of IL-17:The mean of group A is 4.46±0.61ng/L,the mean of group B is 4.49±0.54 ng/L, the mean of group C is 5.01±0.69ng/L, the mean of group D is 5.57±6.69ng/L. The concentrations of IL-17 in experimental C and D are higher than the control group (group A) (P<0.05). Group B and control group is not significant (P>0.05). Group D and group C is significant (P<0.05). The concentrations of Cathepsin K is positive correlation with PD, AL, ABL.Conclusions:1 The concentration of cathepsin K in GCF with chronic periodontitis is higher than in healthy persons, concentration decreased with the increasing periodontitis lesions.2 The concentration of IL-17 in GCF with chronic periodontitis is higher than in healthy persons, concentration increased with the increased periodontitis lesions.3 Cathepsin K and IL-17 can be used as important markers in GCF to reflect the development and the extent of damage of chronic periodontitis. Also can help to observe and determine the progress of periodontitis, and have a great significance of treatment and guidance of periodontitis.
|
PDF Full Text Request