The Relationships Between Tnf-α, IL-12B And IL-10 Gene Polymorphisms And Hepatitis B In Chinese Han Hemodialysis Patients

Aims To provide a comprehensive and reliable tabulation of available data on the epidemiological characteristics and risk factors for hepatitis B virus (HBV) infection in maintenance hemodialysis (HD) patients in China and help to inform prevention programs and guide future research. Methods A systematic review was constructed based on the computerized literature database. 95% confidence intervals (CI) of infection rates were calculated using the approximate normal distribution model. Odds ratios and 95% CI were calculated by fixed or random effects models. Hepatitis B surface antigen positivity (HBsAg (+)) was set as the sign of HBV infection. Results Fifty studies met our inclusion criteria. The pooled prevalence of HBV infection among HD patients in China was 11.9%. Blood transfusion was correlated with an increase in HBV infection (P=0.05). HD patients with a long-term history were more likely to be infected than those with a short-term history. The levels of alanine aminotransferase were higher in the HBsAg (+) patients (P<0.001). Large doses of HBV vaccine (80μg/dose) increased the seroconversion rate. The response rate of intradermal injection of HBV vaccine was higher than that of intramuscular injection. Conclusion Hepatitis B is still one of the main complications in HD patients in China and the frequency of blood transfusion and duration of hemodialysis were the risk factors. Large doses and intradermal injection of HBV vaccine were recommended to prevent HBV infection in HD patients. The findings of this meta-analysis have implications for optimal prevention and treatment of Hepatitis B in HD patients. Aims: The aim of this study was to investigate the possible association of gene polymorphisms of T-helper (Th) 1 and Th2 cytokines and hepatitis B in Chinese Han hemodialysis (HD) patients.Methods: 903 HD patients were included in this study. The genetic polymorphisms in TNF-αpromoter region (-238 and -308), IL-10 gene promoter region (-592 and -819) and 3'untranslated region (3'UTR) of the IL12B (-1188) were studied. Genotyping performed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method.Results: The TNF-α-238 A allele,TNF-α-238, -308 AA haplotype and the IL12B 3'UTR C/C, C/A genotypes were associated with decreased susceptibility to hepatitis B viral infection (P=0.047, P=0.044, P=0.003 and P=0.001), while the GA haplotype of TNF-α-238, -308 was found increased susceptibility to hepatitis B viral infection (P<0.001). And the frequencies of IL-10-592 A/A genotype, IL-10-819 T/T genotype and IL-10-592, -819 AT haplotype were lower in the HBV persistence group (P=0.029, P=0.019 and P=0.048) than those in the virus clearance group.Conclusions: Polymorphisms of TNF-αand IL12B 3'UTR genes may be associated with hepatitis B virus (HBV) susceptibility and polymorphisms of IL-10 genes may be related to the HBV persistent infection in Chinese Han HD patients. Those findings have implications in the optimal prevention and treatment of hepatitis B in HD patients.