Investigate The Relationship Between The Distribution Of Metal Elements From Jiangsu Residents In Vivo And Immunity

Objective: (1) To establish the metal element background values in normal people's blood from Jiangsu Province. (2) To study the effect of lead on human body's immunological function. (3) To explore the factors which effect the blood lead level of workers in the battery plant.Methods: (1) Select the typical areas of Jiangsu Province, from 6 to 55 years non-occupational exposure to chemical factors in the normal population and specific occupational groups. Field surveys include questionnaires and medical examination, collecting blood samples and hygiene surveys.(2) Detect the metals: using inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma mass spectrometer (with CCT mode) the determination of quality control materials provided by the National Center for Disease Control and testing equipment for the Agilent-7500cs. (3) Serum immunoglobulins and complements (IgA, IgG, IgM, C3, C4) Determination:By transmissing immunoturbidimetric assay, the Central Biosino Biotechnology Co.Ltd provided reagents and control materials, testing instruments for the OLYMPUS AU400 automatic biochemical Instrument. Interleukin 2 (IL-2) and tumor necrosis factor (TNF-α) content was determined by Beijing Biotechnology Park Ltd, testing equipment for the KC-Junior microplate reader. (4) Hygiene surveys and determination of Lead on-site in air: According to the GBZ159-2004, on the operating environment of the lead fume and dust is collected. According to GBZ/T160.10-2004, Detecting samples by flame atomic absorption spectrometry.(5) Epidata 3.0 software was used in data entering, and analyzed by SPSS18.0 software. Numerical data were describled by mean and standard deviation, and compared by T-Test or Rank test. Comparison of the multiple mean used analysis of variance. One factor and multivariate analysis used ANOVA analysis logistic regression and multiple linear regression,α=0.05 was chosen as size of all tests.Results: (1) The range of metal elements normal reference value of normal human in whole blood from Typical areas in Jiangsu Province: males Cd(μg/L): 0.12-3.57, Pb(μg/L): 16.7-66.24; women Cd(μg/L): 0.03-1.27, Pb(μg/L): 11.02-52.04; Cs, In and Tl were no gender difference: Cs(μg/L): 0.90-3.79, In(μg/L): 0.01-0.12, Tl(μg/L): 0.01- 0.34. (2) The blood lead levels between exposed group and control group were significantly different (P<0.05). The serum IgG, IgM, C3 and C4 determined were evidently lower than those in control group (P<0.05), while the serum IgA was weak lower than that in control group (P>0.05). The blood lead levels between high-lead group and low-lead group were significantly different (P<0.05). The serum IL-2, TNF-αdetermined in high-lead group were slightly higher than those in control group, but there was no significant (P>0.05). (3) Univariate analysis showed that the worker's smoking,drinking,daily drinking water,washing hands before eating,labor intensity,lead concentration in the air can cause blood lead level difference extently to workers in the battery plant.Multiple linear stepwise regression analysis showed that the high concentrations of lead in the workplace and smoking are the risk factors to the workers.Conclusion: (1) Gender, age, region influence significantly on the content of metal elements in body. the choice of the control group should consider these factors.(2) The qualified rate of the lead concentration in air rate is low. The blood lead level of Workers is relativly high.Generally,the high concentrations of lead in the workplace and smoking are the risk factors to the workers. (3) Lead can inhibit human immune function.