The Expression Of FOXM1 In Laryngeal Squamous Cell Carcinoma, And Its Role In The Inhibition Of The Proliferation, Migration And Invasion Of HEP-2 Cell Line By Curcumin

Objective:To investigate the expression of FoxM1 in laryngeal squamous cell carcinoma(LSCC) and its clinical significance. To investigate the effect of curcumin on the proliferation, invasion and metastasis of Hep-2 cell line. To investigate the expression of FoxM1 in Hep-2 cell line, which is treated with or without curcumin.Methods:Immunohistochemical was used to examine the expression of FoxM1 in 89 LSCC tissues, 89 adjacent normal tissues and 20 laryngeal papilloma(LP) tissues. The relationship between FoxM1 expression and the clinicopathological parameters of LSCC were analyzed. MTT, migration and invasion determinations were used to examine the effect of curcumin on the proliferation, invasion and metastasis of Hep-2 cell line. Reverse transcriptase polymerase chain reaction(RT-PCR) was used to examine the expression of FoxM1 in Hep-2 cell line, which is treated with or without curcumin.Results: FoxM1 expression were gradually decreased from LSCC to LP and normal tissue. The difference was statistical significant(P<0.05). There was a significantly correlation between FoxM1 and histologic differentiation, T stage, lymph node metastasis and clinical stage. Tumors with poorer differentiation, in higher T stage, with lymph node metastasis or in higher clinical stage presented higher FoxM1 expression than tumors with better differentiation, in lower T stage, without lymph node metastasis or in lower clinical stage. Curcumin suppressed the proliferation of Hep-2 cell, and the effect was dose-and time-dependent manners. Moreover,curcumin suppressed the migration and invasion of Hep-2 cell, and the effect was dose-dependent manner. The expression of FoxM1 was down regulated by curcumin ,compared with those without treating by curcumin(P<0.05).Conclusion :The up-regulation of FoxM1may play an important role in the invasion and metastasis of LSCC. Inhibition of the proliferation, migration and invasion of Hep-2 cell by Curcumin maybe through the way of suppressing the FoxM1expression.