The Research On Expression Of Smac Protein And Survivin Protein In Small Intestinal Stromal Tumors

Research BackgroundSmall intestine stromal tumors (SISTs) is one of a common gastrointestinal stromal tumors, its potential malignant degree is high. It is easy to recur postoperation, treatment effectiveness is not so good and prognosis is poor. Therefore, finding a biological behavior indicators to predict its development, to evaluate the potential malignant degree, prognosis and guide therapy is important.ObjiectiveBy detecting the the expression of Smac protein and Survivin protein in SISTs, peritumorial tissues of SISTs, normal intestinal tissues, investigate the relationship with the clinic pathological parameters in SISTs, and explore the roles or pathogenesis in SISTs. Thus provide an evidence for assessment the latent malignancy classification and offer a new idea for the treatment of SISTs.MethodsImmunohistochemical method was used to detect the expression of Smac protein and Survivin protein in 57 cases SISTs,42 cases peritumorial tissues of SISTs,30 cases normal intestinal tissues and investigate the relationship with the clinic pathological characteristic in SISTs.Results1. The positive expression rate of Smac protein in SISTs peritumorial tissues of SISTs and normal intestinal tissues were 36.84%, 3.33%,93.33% respectively. The positive expression rate of Smac protien in SISTs were significantly lower than peritumorial tissues (p< 0.01) and normal intestinal tissues (p<0.01). there was no statistical significance between normal intestinal tissues and peritumorial tissues (p >0.05).2. The positive expression rate of Survivin protien in SISTs, peritumorial tissues of SISTs were 73.68%,7.14% respectively, there is no expression in normal intestinal tissues. The positive expression rate of Survivin protien in SISTs was significantly higher than in peritumorial tissues(p<0.01) and normal intestinal tissues(p<0.01). There was no statistical significance between the normal intestinal tissues and peritumorial tissues(p>0.05).3. The expression of Smac protein and Survivin protein in SISTs were statistically related with tumor diameter, Karyokinesis and the latent malignancy classification(p<0.05), but had no relationship with age, gender and tumor location(p>0.05).4. The expression of Smac and Survivin in SISTs were negative correlation(p<0.05), r=-0.287.Conclusions:1. Smac protein was highly expressed in normal intestinal tissues and low expression in small intestinal stromal tumors, Survivin protein was highly expressed in small intestinal stromal tumors and low expression in normal tissuess, suggesting that Smac and Survivin may be related to the occurrence and development of SISTs.2. The expression of Smac protein in SISTs reduced with increaseing the malignant potential grade and tumor max diameter, Survivin protein expression increased with lowering the classification of malignant potential and decreasing tumor max diameter, suggesting that Smac and Survivin can be detected together to be an reference to judge the latent malignancy degree and prognosis of the SISTs.3. Smac and Survivin each other may have feedback adjustment in the development of SISTs, Smac and Survivin may become new targets for therapy.