The Effects Of Progesterone On Nogo-A,GFAP And GAP-43 Protein Expression In Rat Brain After Traumatic Brain Injury

Obiectives:To detecting the traumatic brain injury and progeste-rone interfere with NogO-A, GFAP and GAP-43 Protein expression in rat brain. Approach progesterone for protective effects of nerve after traumatic brain injury in rat.Methods:75 healthy Sprauge-Dawley male rats are divided randomly into five groups:the sham-operation group(n=25),the model group(n=25) and the treatment group(n=25). Each group is divided into five time points,which are 1 day,3 days,7 days,14 days and 28 days. The rats of model and treatment group as model of moderate cerebral contusion suffered traumatic brain injury by Freeney method, the sham-operation group only scalp incision and open bone window, but not do blow. The treatment group by intraperit-oneal injection of progesterone (10mg/kg/d) after six hours, sham operated rats injected corresponding dose of NS in the same time phase. These brain injured rats are executed at the time points above-mentioned. The patholo-gical changes are observed by HE drum dyeing, and Immunohis tochem-istry are used to detect the the expression of NogO-A,GFAP and GAP-43 protein.Results:1. Change of pathological section:HE staining showed:Sham-operation group rats brain structure is clear, even and compact. The structure of model group's rats brain disoders, local hemorrhage and edema, inflamm-atory cell infiltration can be seen in trauma areas, showing nuclear condensation or dissolution.Treatment group compare with the model, the structure of rats brain also disoders, swelling of brain tissue injury is mild, neuronal degeneration is also less.2. The Nogo-A protein expression of model group rat brain tissue is higher than the sham group in 1d,3d,7d,14d and 28d.(P<0.05),the expres-sion reached the peak in the 14d, then decreased gradually.The expression is still higher than normal level in the 28th day.The expression of Nogo-A protein in treated rat brains is lower than the model group at each time point (P<0.05).3. The GFAP protein expression of model group rat brain tissue is higher than the sham group in 1d,3d,7d,14d and 28d(P<0.05).The expression reached the peak in the 14d, then decreased gradually.The expression is close to normal level in the 28th day. The expression of GFAP protein in treated rat brains is lower than the model group at each time point (P<0.05).4. The GAP-43 protein expression of model group rat brain tissue is higher than the sham group in 1d,3d,7d,14d and 28d.(P<0.05),the expression reached the peak in the 14d, then decreased gradually.The expression is still higher than normal level in the 28th day.The expression of GAP-43 protein in treated rat brains is higher than the model group at each time point (P<0.05).Conclusions:1.The rat brain expression of Nogo-A protein is increase at first and then decrease after traumatic brain injury, suggesting that the role of neuroprotective is gradually weakened after enhance.2. The rat brain expression of GFAP protein is increase at first and then decrease after traumatic brain injury, suggesting that the role of neuropro-tective is gradually weakened after enhance.3. The rat brain expression of GAP-43 protein is increase at first and then decrease after traumatic brain injury, suggesting that the role of promote neuroprotective is gradually weakened after enhance.4. Progesterone has neuroprotective effects after traumatic brain injury in rats, by reducing the expression of Nogo-A and GFAP around the lesion, enhance the expression of GAP-43, to reduce brain injury and promote nerve regeneration after injury.