Studies On The Chemical Constituents And Bioactivities Of Emmenopterys Henryi Oliv

Emmenopterys henryi Oliv (Rubiaceae) is one of the two species of the genus Emmenopterys Oliv, but the only one in China according to . Emmenopterys henryi Oliv occurs in southwest and Yangtze valley of China is large deciduous arbor, and it has white or yellew flower, so it is planted as ornamental in the gaden.The roots and barks of this plant, a Chinese folk medicine, are efficacious in therapying gastropathy.A systematically phytochemical study was carried out, which led to the isolation and identification of thirty-eight chemical constituents including alkaloids, iridoid glucosides, coumarins, lignans, triterpenoids, steroid etc. Among these compounds, two compounds are new (xgs-1 , xgs-11 and xgs-38). Their structures were elucidated on the basis of spectral data including 1H-NMR, 13C-NMR, DEPT, 1H-1HCOSY, NOESY, HMQC, HMBC, ESI-MS, IR, as follows: xgs-1: 4-methyl-5-oxostrictosidine xgs-2: 5-oxostrictosidine xgs-3: loganin xgs-4: loganic acid xgs-5: 6'-O-β-D-apiofuranosylsweroside xgs-6: sweroside xgs-7: vogeloside xgs-8: grandifloroside xgs-9: cleomiscosin D xgs-10: diospyroside xgs-11: 8-O-(6'-O-β-D-apiofuranosyl-β-D-glucopyranosyl)-6-methoxy-7-hydroxycoumarin xgs-12: xeroboside xgs-13: haploperoside A xgs-14: scopolin xgs-15: fraxin xgs-16: scopoletin xgs-17: trans-caffeic acid-4-O-6'-O-(3",5"-dimethoxy-4"-hydroxybenzoyl) -β-D-lucopyranoside xgs-18: tortoside F xgs-19: 5-methoxybalanophonin xgs-20: balanophonin xgs-21: (+)-lyoniresinol-3α-O-β-D- glucopyranoside xgs-22: (-)-lyoniresinol-3α-O-β-D- glucopyranoside xgs-23: (+)-lyoniresinol-3α-O-9-O-(6"-3,5-dimethoxy-4-hydroxybenzoyl) -β-D- glucopyranoside xgs-24: 3,4,5-trimethoxyphenyl-O-β-D-apiofuranosyl-(1→6)-O-β-D- glucopyranoside xgs-25: isocupressic acid xgs-26: 13-epicupressic acid xgs-27: clethric acid xgs-28: pomolic acid xgs-29: ursolic acid xgs-30: ursolyl acetate xgs-31: taraxerol xgs-32: 6β-hydroxy-sitost-4-en-3-one xgs-33:β- sitosterol xgs-34: sitost-4-en-3-one xgs-35: 3-hydroxymethyl-4-vinylhexane-1,6-diol xgs-36: sucrose xgs-37: octadecanioc acid xgs-38: 3-methoxy-4-methyl-5,6-dioxostrictosidineThe ethanol extract, EtOAC, n-BuOH fraction and some compounds were evaluated for cytotoxicity MTT assay, a short term in vitro test.The results indicated that n-BuOH fraction and compounds xgs-1, xgs-2, xgs-38 showed cytotoxic effect against 4 human cancer cell lines, A549, COLO-205, QGY-7703 and THP-1. In anti-inflammatory activity test of ethanol extract, two extractive fractions and some compounds, the results of anti-inflammatory activity showed ethanol extract, n-BuOH fraction and compounds xgs-1, xgs-2, xgs-3 displayed inhibitory ability against LPS-induced NO production in RAW 264.7 macrophages.