Study On The Expression Of Adrenomedullin In The Renal Cancer Tissue And Its Correlation With The Microvessel Density

Objective: Renal cell carcinoma (RCC) is the leading cause of death in urinary system tumors. In recent years its morbidity and mortality in the continuing rise, so increase the pathogenesis of renal cell carcinoma, clinical treatment for the ultimate objective to provide effective indicators.The role of the angiogenesis in the renal cancer development has been identified. And the adrenomedullin (ADM) is a new vasoactive peptide. Through the immunohistochemistry , the expression of adrenomedullin in the renal cancer and its correlation with the microvessel density is to be observed to provide a reliable molecular-biological indicator and theoretical basis for the clinical treatment of renal cancer.Method: collected the renal cancer operation cases with complete data from the urinary surgery department of HeBei University second hospital from April 2009 to September 2009 and the total case number was 33. Before the operation, the patient did not receive any anti-tumor therapy. The operation samples were obtained in vitro under the aseptic condition. And the samples included the tumor tissue and the normal renal parenchyma tissue whose distance to the tumor edge was over 5cm. There were two samples whose diameter was about 1cm. Both were fixed by 10% methanol.Result: Tumor cells with obvious ADM marker in the renal cancer tissue would be detected using the immunohistochemistry method. The ADM mainly expressed in the cytoplasm and its positive rate was 100%, while the positive expression rate in normal tissue was 9.1%. which indicated that the ADM expression level in the renal cancer tissue was different from that in the normal renal tissue.The microvessel density in the renal cancer tissue was 43.39±7.81, while the microvessel density in the normal tissue was 9.73±4.22. In the nonparametric test, the Z value was -5.014 and the two-side probability P was equal to 0.000 less than 0.05. So refused H0 and accepted H1, which indicated that the microvessel density in the tumor tissue was different from that in the normal tissue and the microvessel count was obviously higher than that in the normal tissue. In the immunohistochemistry detection, the microvessel density in the renal cancer tissue with high expression of ADM was 43.78±7.61, while the microvessel density in the renal cancer tissue with low expression of ADM was 10.35±5.53. In the variance test, F was equal to 420.266 and P was 0.000 less than 0.05. So refused H0 and accepted H1. And it was considered that the high or low expression of ADM in the renal cancer tissue was correlated with the microvessel count. The higher the ADM expression, the larger the microvessel density value was.Conclusion: After comparing the tumor tissue with the normal one in 33 renal cancer patients and through the immunohistochemistry detection, this study comes to an conclusion that ADM has high expression in renal cancer tissue while its expression in certain normal tissue is low, and the high expression of ADM is correlated with the number of microvessel density. This indicates that, as a biological indicator, ADM plays a certain role in the occurrence and development of renal cancer. After further study on the property of ADM, it might provide a theoretical basis for the pathogenesis of renal cancer and the comprehensive therapy, thus making better clinical treatment for the renal cancer.