| Objective:By animal in vitro and in vivo, study of sodium selenite alone and with Trisodium phosphate combined application of arsenic poisoning of protection and its mechanism, for Sodium selenite on prevevtion and treatment of Arsenic poisoning provied new ideas. Methods:(1)In vitro, Using primary mouse liver cell culture technology, After processed cell by certain dosages of NaAsO2, different dosages of Sodium selenite and Trisodium phosphate were added to nutrient fluid, then through MTT, measured OD, Study influnce of sodium selenite alone or combined with Trisodium phosphate of arsenic exposure in mice on multiplication-inhibition of liver cells. (2)In vivo, SPF Kunming mice were 70, were randomly divided into seven groups: Control group, Arsenic in the model group, Sodium selenite control group, Trisodium phosphate control group, Sodium selenite Intervention group, Trisodium phosphate Intervention group, Sodium selenite+ Trisodium phosphate Intervention group, after 60 days, the animal were sacrificed and a series of biochemical indices were detected on organs. By xanthine oxidase activity of superoxide dismutase(SOD), thiobarbituric assay of malondialdehyde (MDA) content, ultraviolet spectrophotometry Detection of myeloperoxidase (MPO) content. (3)use(HPLC-HGAFS), determination of liver and kidney content of MMA and DMA. Results:(1)In vitro, low and medium dose(3μmol/L,5μmol/L,10μmol/L)Sodium selenite Intervention group OD was significantly higher than Arsenic in the model group(P<0.05). Sodium selenite+ Trisodium phosphate Intervention group OD was significantly higher than other groups(P <0.05). (2)In vivo, Arsenic exposure in mice have different degrees of weight loss, organ coefficient reduced; Arsenic group mouse liver, kidney SOD, MPO levels lower than the control group(P<0.05), MDA content was higher than the control group(P< 0.05); Sodium selenite Intervention group the weight Significantly higher than Arsenic in the model group(P< 0.05); MDA lower than Arsenic in the model group(P<0. 05);Sodium selenite+ Trisodium phosphate Intervention group the weight Significantly higher than Arsenic in the model group(P<0.05), Organ coefficient lower than Arsenic in the model group(P<0.05), DMA,MMA lower than Arsenic in the model group(P<0. 05); Sodium selenite Intervention group of Indicators and Sodium selenite+ Trisodium phosphate Intervention group of indicators No difference(P>0.05)Conclusions:(1)low doses of sodium selenite(3μmol/L)of arsenic exposure in mice can promote the proliferation of liver cells, High doses of sodium selenite(25μmol/L)of arsenic exposure on liver cell proliferation without promoting the effect. No synergy on Sodium selenite and Trisodium phosphate for arsenic exposure on liver cell proliferation. (2)Sodium selenite on sodium arsenite in mice liver and kidney lipid peroxidation damage has obvious protective effect. No synergy on Sodium selenite and Trisodium phosphate for Liver and kidney lipid peroxidation, (3)Sodium selenite on arsenic poisoning has a protective effect.
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