The Significance Of P53 Gene And FHIT Gene Union Examination In Lung Cancer Early Diagnosis

Objective Researching the normal bronchial mucosa,atypical hyperplasia, carcinoma in situ, infiltration carcinoma and benign lesions the p53 gene mutation and FHIT gene deletion by using AFB(autofluorescence bronchoscopy) in order to study their role in the development of lung cancer and to investigate of P53 gene mutation and FHIT gene deletion in lung cancer screening and early diagnosis value.Methods Collected use since fluorescent bronchoscopy biopsy of 27 normal bronchial mucosa cases, 74 atypical hyperplasia cases, 23 carcinoma in situ cases, 161 infiltrating carcinoma cases and 32 benign lesions fresh specimens cases, application polymerase chain reaction (PCR) and DNA sequencing technologies detection p53 gene mutation, application retrovirus polymerase chain reaction (PCR) and RT - DNA sequencing technologies detection FHIT gene expression missing.Results p53 gene in normal bronchial mucosa , atypical hyperplasia, carcinoma in situ, infiltration carcinoma and benign lesions, the mutation rate were 0%,27.0%,52.2%,67.7%,0%, respectively. The FHIT gene expression missing rate were 0%,55.4%,65.2%,66.5%,9.4%, respectively. The positive rate of combined detection of the two genes were: 0%,56.8%,86.9%,90.7%,9.4%. Atypical hyperplasia and carcinoma in situ tissue p53 gene mutation was significantly (P﹤0.05),Atypical hyperplasia, carcinoma in situ, infiltration carcinoma tissue FHIT gene deletion was no significant difference (P﹥0.05),The infiltration cancer organizes the p53 gene mutation and the FHIT gene deletion the difference has statistics significance in the cellule lung cancer with the non-cellule lung cancer between(P﹤0.05). The organization pathology classification different infiltration cancer organizes the p53 gene mutation and the FHIT gene deletion difference has statistics significance(P﹤0.05).Conclusion p53 gene mutation and FHIT gene deletion in lung cancer and atypical hyperplasia is a frequent event in the development of lung cancer plays an important role. Two gene union examination may enhance the lung cancer diagnosis the sensitivity and the specificity, has the important meaning to the lung cancer early diagnosis.1. The p53 gene elevates gradually along with the tissue cell malignant degree's aggravation rate of mutation, the p53 gene mutation possibly mainly occurs in the preinvasive carcinoma stage. p53 gene mutation in lung cancer is a frequent event in the development of lung cancer plays an important role, and it may be an early molecular events in lung cancer. The p53 gene mutation is the important factors contributed to tumor formation. Detection mortem mirror biopsy specimens p53 gene mutation can be used for lung cancer early and pre-cancerous diagnosis.2. The FHIT gene elevates gradually along with the tissue cell malignant degree's aggravation rate of mutation, the FHIT gene deletion possibly mainly occurs in the atypical hyperplasia stage. FHIT gene deletion in lung cancer is a frequent event in the development of lung cancer plays an important role, and it may be the early starting link which the lung cancer occurs. The FHIT gene deletion is the important factors contributed to tumor formation. Detection mortem mirror biopsy specimens FHIT gene deletion can be used for lung cancer early and pre-cancerous diagnosis.3. Union examination the p53 gene mutation and the FHIT gene deletion may enhance the lung cancer diagnosis the sensitivity and the specificity, has the important meaning to the lung cancer early diagnosis.