Study Of Toxic Mechanism Of Anthraquinone Derivatives Binding DNA By The Resonance Light Scattering

DNA intercalation theory is introduced to study the interaction anthraquinone with DNA by resonance light scattering spectroscopy. The aim is to reveal potential toxic mechanism of anthraquinone. Meanwhile we investigate pharmacological action of anthraquinone when environmental conditions or factors change. We start a new research to study pharmacological action of medicine and avoid the toxicity. The results show that:1. Resonance light scattering technique has been studied potential toxic mechanism of physcion, chrysophanol, rhein and barbaloin interactions with DNA, which are based on their interactions with DNA and compared with ethidium bromide (EB), adriamycin and mitoxantrone. And then, we construct evaluation methods of anthraquinone derivatives's potential toxicity. The saturation value binding with DNA, which is calculated by the resonance light scattering results, is first put forward as an indicator to evaluate the ability of intercalating into DNA and the potential toxicity of anthraquinone molecule. The greater the saturation value is, the stronger the ability of intercalating into DNA and the potential toxicity of anthraquinone molecule are. The results show that the saturation value of physcion, chrysophanol, rhein, barbaloin, mitoxantrone, adriamycin or EB interacting with DNA are 0.15, 0.53, 0.66, 1.6, 3.31, 10.58, 14.70, respectively. From these results, it can be speculated that physcion, chrysophanol, rhein and barbaloin show a weak capacity of intercalating into DNA and toxicity, but they are much lower than those of mitoxantrone, adriamycin and ethidium bromide.2. We study the interaction of chrysophanol with DNA to reveal the toxic mechanism of chrysophanol by the saturation value. Its saturation value changes when environmental conditions or factors changes, such as amino acids, sodium chloride and glucose. Compared with the saturation value of chrysophanol-DNA-pH7.4-System, the change rate of the saturation value binding with DNA of sodium chloride, glucose, pH3.5 and pH8.1 on chrysophanol- DNA-pH7.4-System are +257%, -36%, -83%, -51%, respectively. The change rate of the saturation value binding with DNA of alkaline histidine and acid aspartate are -66% and +181%, respectively. Non-polar amino acids has no effect on chrysophanol-DNA-pH7.4-system.3. We study the interaction of rhein with DNA to reveal the toxic mechanism of rhein by the saturation value. Its saturation value changes when environmental conditions or factors changes, such as amino acids, sodium chloride and glucose. Compared with the saturation value of rhein-DNA-pH7.4-System, the change rate of the saturation value binding with DNA of glucose on rhein-DNA-pH7.4-System is -12%. The change rate of the saturation value binding with DNA of alkaline histidine, acid aspartate and tyrosine are +33%, +186%, -42%, respectively. Non-polar amino acids has no effect on rhein-DNA-pH7.4-system.