Study On Potential Toxic Mechanism And Evaluated Methods Of Anthraquinones Madder Color

The present study was the first to use the fluorescence probe method, ultraviolet visible absorption spectroscopy(UV-vis), DNA thermal denaturation experiment, viscosity experiment, cyclic voltammetry(CV), and resonance light scattering(RLS) to study the interaction between DNA and three anthraquinones madder pigments: Lucidin, Rubiadin, and Anthragallol. RLS was used to calculate the combined with saturated value of three kinds of anthraquinones madder pigment with DNA when p H=7.41. In order to quickly evaluate potential toxicity of Lucidin, Rubiadin, and Anthragallol, we developed a new method based on the interaction with DNA and the DNA saturation with adriamycin, alizarin, chrysophanol, and rhein as references. The main research results are as follows:1. We used the fluorescence probe method, UV-vis, DNA thermal denaturation experiment, viscosity experiment, and cyclic voltammetry to explore the three kinds of medicines interact with DNA. The fluorescence-quenching phenomenon between three kinds of medicine molecules and DNA is similar to EB via fluorescence probe method. UV-vis spectrometry demonstrated that redshift and hypochromic effect occurred between three kinds of drug molecules and DNA. DNA thermal denaturation experiments established that these three kinds of drug molecules can enhance Tm(DNA chain solution temperature). Electrochemical tests showed that these three substances produce electrochemical reaction with DNA and decrease the peak value of ethidium bromide. Taken together, these results indicate that insertions exist between the three kinds of material and DNA. In this study, three kinds of drug molecules quenching form were obtained by Stern-Volmer equation and its combination constant of the EB-DNA system was calculated under different temperature conditions. The value of n and r of different concentrations of three kinds of drug molecules with the EB-DNA system were calculated by Scatchard equation. From there, the combination ability and bonding strength between three kinds of molecules—Lucidin, Rubiadin, and Anthragallol—and DNA can be expressed visually.2. We used RLS to calculate combined with saturated value of Lucidin, Rubiadin, and Anthragallol with DNA in the neutral environment.3. Based on the RLS, compared with the saturated value of adriamycin, chrysophanol, rhein, and alizarin to calculate the gradient of Lucidin, Rubiadin, and Anthragallol. The combination of the three substances saturation value was lower than adriamycin but was higher than chrysophanol, rhein, and alizarin. These results point to the conclusion that the level of potential toxicity of these three drug molecules is lower than adriamycin but higher than chrysophanol, rhein, and alizarin.