Study Of Potential Cytotoxicity In Rhubarb Anthraquinone Derivatives And The Mechanism Of Rhubarb De-toxicity By Compatibility Based On Spectroscopy

In order to study the mechanism of the potential toxicity of rhubarb anthraquinone derivatives and its application in studying the detoxicity mechanism of relative Chinese Medicine by compatibility, in this paper, DNA intercalation theory is introduced to study the interactions between the molecular of rhubarb anthraquinones with DNA molecule by fluorescence and resonance light scattering spectroscopy, and also the effects of DNA and rhubarb anthrquinone on different conditions, meanwhile, research the antibacterial property of rhubarb anthraquinone in escherichia coli, staphylococcus aureus and bacillus subtilis. It can be found that the three rhubarb anthraquinone derivatives have varying degrees of sterilization in a certain concentration range; the interactions between emodin(EM), rhein(RE) and 1,8-dihydroxyanthraquinone(DAQ) with DNA can make their own resonance scattering signal enhanced, and the phenomenon was similar to the interactions of EB with DNA; The intensity of the fluorescence peak of EM and DAQ were increased with addition of DNA, and their binding constants were calculated calculated KA(EM) = 0.18×105 L·mol-1, KA(DAQ)=0.19×106 L·mol-1 respectively; The interactions of RE with DNA result in the endogenous fluorescence static quenching of RE. The binding constant of the static quenching was calculated KA(RE)=0.727×104 L·mol-1. From the point of view of DNA intercalator theory, these may be the reasons of pharmacological action or cytotoxicity of rhubarb anthraquinone derivatives. And we also found that the interaction of EM and DNA was influenced by different solution conditions. RLS intensity enhances on low pH value and polar amino acids. Contrarily, nonpolar amino acids, sodium chloride, glucose can decrease RLS intensity. The alteration in environmental factors has a significant change in RLS intensity of EM-DNA system. Therefore, the changing environmental factors can affect the interaction between AE and DNA, so as to achieve the purposes of attenuation and synergistic effect.From the results above, it could be presumed that these intercalating relationships between rhubarb anthraquinone molecules and DNA molecules may be the foundation of showing the physiological activities of rhubarb, also may be the cause of the potentially toxicity of rhubarb. And these intercalating relationships can be affected by environmental factors. Therefore, we can change the environmental factors to increase the efficacy of rhubarb anthraquinone derivatives or reduce the toxicity of aloe anthraquinone derivatives. The spectroscopy results and methods based on studying rhubarb anthraquinone interacting with DNA and the environment influence introduced by this article and from the point of view of DNA intercalator theory to consider the physiological activities and potentially toxicity of rhubarb, could start a new research exemplification to study the drug effects of traditional Chinese medicine and the potential toxicity mechanism and then, to study the compatibility attenuation and synergistic effect of Chinese medicine and also the way to avoid the toxic and side effects.