The Effects Of Pentoxifylline On Apoptosis Of Myocardial Cell And Inflammatory Factors In Heart Failure Rats Triggered By Pressure Overload

Objective: To investigate the effects of pentoxifylline on apoptosis of myocardial cell and inflammatory factors in heart failure rats triggered by pressure overloadMethods: Pressure overload induced by abdominal aortic banding. Thirty-five male SD rats were subjected to five groups at random: sham-operation group, model group, aminophylline group, pentoxifylline-low group, pentoxifylline-high group. The left ventricular and function indexes of five groups were observed by high-frequency echocardiography on the fourth and the eighth week. Then, in the eighth week, we calculated the heart weight indexes and left ventricular weight indexes of rats, observed the change of the myocardium histomorphology, and detected apoptotic cardiomyocyte using the terminal deoxynucleotidy1 transferase mediated Dutp nick end labeling method(TUNEL). The expression of inflammatory cytokine from myocardium were detected respectively by biochemical methods, and immunohistochemistry and Western blot.Results: 1. the left ventricle posterior wall thickness (LVPWd) of model group, aminophylline group, pentoxifylline-low group and pentoxifylline-high group in the fourth and eighth week were significantly higher than that of ham operation group (P<0.05). In the eighth week, the percent fraction shortening (FS) of model group and aminophylline group were significantly lower than before (P<0.05). And the percent fraction shortening (FS) of model group, aminophylline group, pentoxifylline-low group and pentoxifylline-high group were significantly lower than that of ham operation group (P<0.05). In the eighth week, percent ejection fraction (EF) of pentoxifylline-low group and pentoxifylline-high group were significantly higher than that of model group and aminophylline group (P<0.05), though those of model group and aminophylline group were similar, and the EF of model group and aminophylline group were significantly lower than that of ham operation group (P<0.05). The LVPWd of pentoxifylline-low group and pentoxifylline-high group were significantly lower than that of model group and aminophylline group (P<0.05). In the eighth week, the EF of pentoxifylline-low group and pentoxifylline-high group were similar with before, and through those of pentoxifylline-low group and pentoxifylline-high group were similar. The left ventricular end diastolic diameter, left ventricular end systolic diameter, interventricular septal thinckness of the five groups were similar.2.Hematoxylin-eosin staining and Western blot show: cadiocyte degenerated, hypertrophy, focal necrosis, arranged confused and companied with inflammatory cells in pentoxifylline-low group and pentoxifylline-high group. Pathohistology of the two groups lied between model group and ham operation group.3. The detection of TUNEL: Apoptotic indexes of pentoxifylline-low group and pentoxifylline-high group were significantly lower than that of model group and aminophylline group (P<0.05). In those of pentoxifylline-low group and pentoxifylline-high group were similar. 4. The detection of biochemical methods and immunhistochemistry and Western blot: the expressions of TNF-α, IL-6 in pentoxifylline-low group and pentoxifylline-high group were significantly lower than that of model group and aminophylline group (P<0.05), and the expressions of IL-10 in pentoxifylline-low group and pentoxifylline-high group were significantly higher than that of model group and aminophylline group (P<0.05), In those of pentoxifylline-low group and pentoxifylline-high group were similar.Conclusion: PTX can regulate the inflammatory cytokine of myocardium, suppress the apoptosis of myocardial cell, postpone the remodeling of ventricular, and improve heart function of heart failure rats.