Analysis And Identification Of Fritillaria By Using Infrared Spectroscopy Method

Bulbus Fritillariae (in Chinese named Beimu), referred to the bulbs of several Fritillaria species (Liliaceae), is a commonly used anti-tussive and expectorant herb in traditional Chinese medicine (TCM) for more than 2000 years which is also used as a dietary supplement in China.Because there are many varieties of herbal medicine, which are the same as Beimu belong to Fritillaria, they are quite similar, and different parts and producing areas of Beimu have diverse chemical constituents and drug effects. Therefore, rapid and accurate identification method can play a significant role on guaranteeing their curative effect. Infrared spectroscopy (IR) is one of the routine means to distinguish chemical compounds and confirm material structures. The IR spectra of Traditional Chinese Medicine (TCM) include spectra of each compound in the system, which shows the whole information of TCM.In this thesis, we have analyzed and identified the quality of Beimu via the multi-steps IR macro-fingerprint method and combined with the software of Computer Analysis Comparison. The main conclusions include:(1) Feral Bulbus Fritillariae Cirrhosae(CB) and feral Bulbus Fritillariae Ussuriensis(UB) of different origins are quite different, the reason of which is that they have quite different grown conditions of climatic conditions, topography, soil and so on. So, these distinction leads to the content of their chemical components. Beimu in the same province are quite similar but not the same. It is thus clear that the influence of the kind of Beimu chemical components by the different origins is not so big while it is big in the content accumulation. (2) The IR spectra of the different kinds of Beimu materials shows that CB and Bulbus Fritillariae Thunbergii (TB) both have a obvious peak at 1647 cm-1 while the peak of other three Beimu is at 1654 cm-1. In the second derivative IR spectra, CB has the strongest peak at 1647 cm-1, TB changed into two peaks at 1640 cm-1 and 1628 cm-1, while other three Beimu are quite similar at 1638cm-1 and 1627cm-1. The three peaks characteristic intensity in the region at 1200-1300cm-1 of the 2D-IR spectra correspond the stretching of the -C-O-C-groups. (3) The research of three different CB:Songbei(SB), Qingbei(QB), Lubei(LB) shows that the different parts of the bulbs contains different active principle. The bud and scale leaf of SB contain dissimilitude active principle with QB and LB. The bud also contains the same starch and active principle with the bulb. So, this is consistent with the rules of pharmacopeia that not remove the bud of CB when using them. Meanwhile, it is also identified that the quality of SB is much better than the other two. (4) The starch contained in the original medicinal extracts has greatly reduced, while the relative content of the effective components, such as the total alkaloid and saponins, has increased. The IR spectrums of the aqueous and alcohol extract of F. cirrhosae are distinctly different with the relatively peak intensity with the other Beimu at 1100cm-1 and 1630cm-1. And We can find peaks at 1120±5 and 618cm-1 in the IR spectrum of Beimu aqueous extract which are the characteristic absorption peaks of the sulfate, and this is related to the use of sulfur fumigation in the processing. And it is also show that the sulphur residue in Beimu can be identified by the FTIR.In a word, the multi-steps IR Macro-Fingerprint method and combined with the software of Computer Analysis Comparison have great applying foreground in the quality control of TCM area.