Effect Of Quercetin And 17-AAG On Radiosensitivity Of HeLa Cells

1 Objective:To investigate the anti-proliferation of Quercetin and 17-AAG on human Uterine Cervix Cancer HeLa cells,to evalute the enhancement of radiosensitivity by Quercetin or 17-AAG on the cells;To investigate the cell cycle change by Quercetin or 17-AAG on the cells.2 Mehtods:HeLa cells were cultured in differect concentrations of Quercetin and17-AAG in vitro.The inhibitory ratio of the cells were measured by MTT assay.Colon forming assay is carried out to study the enhancement of radiosensitivity by Quercetin and 17-AAG on HeLa cells.The cell cycle change was investigated using flow cytometer.3 Results:1) Quercetin could inhibit the growth of the HeLa cells in vitro significantly .The suppression was both in the dose-dependent and time-dependent. Colon forming assay showed that there was a significant difference between HeLa cells treated by different concentrations quercetin and different irradiation doses(P<0.01). The low-dose zone curve became smaller and linear slope increased significantly. The survival rate of all dose points was lower than the control group, and it indicated quercetin had a significantly radiosensitivity effect on the HeLa cells.2)The inhibition of 17-AAG on the HeLa cells was in the dose-dependent and time-dependent. Colon forming assay showed that there was a significant difference between HeLa cells treated by different concentrations 17-AAG and different irradiation doses(P<0.01). The low-dose zone curve became smaller and linear slope increased significantly. The survival rate of all dose points was lower than the control group, and it indicated 17-AAG had a significantly radiosensitivity effect on the HeLa cells. 3) The inhibition of quercetin combine with 17-AAG on the HeLa cells had some synergistic effect. The survival rate of HeLa cells of different combination groups was significantly lower than other groups(P<0.01). The combination groups curve became smaller and linear slope increased significantly. The survival rate of all dose points was lower than other groups, and it indicated the group of quercetin combined with 17-AAG had a synergistic effect radiosensitivity on the HeLa cells.4)Redistribution of Hela cells into a radiosensitive phase of the cell cycle does not account for the quercetin-mediated and 17-AAG-mediated enhancement in radiation-induced cell killing.4 Conclusions:Quercetin and 17-AAG could inhibit the growth and enhanceand the radiosensitivity of the HeLa cells, quercetin combine with 17-AAG definitly enhanced radiosensitivity of the HeLa cells. Redistribution of Hela cells into a radiosensitive phase of the cell cycle does not account for the quercetin-mediated and 17-AAG-mediated enhancement in radiation-induced cell killing.