The Associated Research On The Expression Of RUNX3 MRNA And Protein In Hepatic Cell Carcinoma As Well As RUNX3 Protein Expression In Various Kinds Of Hepatic Tissue

Objective To investigate the expression of RUNX3 mRNA and protein in hepatic cell carcinoma (HCC)and surrounding normal tissue,to analyze the relationship between RUNX3 expression and clinical pathological parameters.Furthermore,to study the expression of RUNX3 protein in 6 kinds of hepatic tissues.Methods Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry(IHC) were performed to detect the expression of RUNX3 mRNA and protein in HCC and surrounding normal tissue respectively, and their relationship with clinical pathological parameters were analyzed.Furthermore, IHC was performed to detect the expression of RUNX3 protein in various kinds of hepatic tissues.Results The relative expression value of RUNX3 mRNA found in 51 cases HCC was 0.4509±0.0963. and that did in 51 cases surrounding normal tissue was 0.9147±0.0222. The difference of RUNX3 expression between two kinds of samples was statistically significant (t=33.6087, p＜0.001). The positives rate of RUNX3 protein expression found in 51 cases HCC tissue was 49.02%(25/51) and that did in 51 cases surrounding normal tissue was 82.35% (42/51).The difference of RUNX3 protein expression between two kinds of samples was statistically significant (x 2=12.5706, p<0.005) The difference of RUNX3 mRNA and protein expression in some clinical pathological parameters involving differentiation degree, invasion, cancer thrombus and diversion in liver were statistically significant (p<0.05).However that were not in another clinical pathological parameters involving gender, cancer diameter, cancer location as well as hemorrhage and necrosis of cancer, histotype (p>0.05) The positives rate of RUNX3 protein expression in various kinds of hepatic tissue can be described as follows:The positives rate of HCC, hepatic hyperplastic nodule,hepatic cirrhosis tissue was 49.02%,78.26%,87.50% respectively.However that of type B hepatitis tissue,hepar adiposum tissue,normal hepatic tissue were all 100%.There was statistics variance between the former three and the latter three respectively but not among the latter three.Conclusion The expression of RUNX3 mRNA and protein in HCC were significantly lower than that in surrounding normal tissue. The lower expression of RUNX3 mRNA and protein in HCC were correlated with some clinical pathological parameters involving differentiation degree, invasion, cancer thrombus and diversion in liver. However that were not with another clinical pathological parameters involving gender, cancer diameter, cancer location as well as hemorrhage and necrosis of cancer, histotype. It was possible that RUNX3 protein expression in the process of HCC was as decrease gradually as progress from precancerous lesion to carcinoma.The gradual decrease of RUNX3 protein expression was correlated with generate and progress of hepatoma. The RUNX3 gene may be an anti-oncogene of HCC.