The Effect Of Progesterone On The Expression Of Connexin 43 In Mouse Premature Uterine Smooth

Objective Observation of progesterone on mouse uterine connexin 43 (Cx43)in the expression.Methods Selected 26 successful pregnancy C57BL/6 female mice were divided into three groups A, B and C.The does of group A was inserted subcutaneously into the back skin of mice of 0.4ml tea oil and intubated with 20% of ethanol 1ml one-time;The does of group B was subcutaneous injection of 25% of the progesterone tea oil solution 0.4ml and intubated with 20% of ethanol 1ml one-time;The does of group C was inserted subcutaneously into the back skin of mice of 0.4ml tea oil and intubated with lml Sodium Chloride(NS)one-time.Western blot with uterine smooth muscle tissue of each group of total protein and phosphorylation of Cx43 protein variation;Records of assessment delivery time for statistical analysis.Results(1)Average gestational age of pregnant rats in the group A (17.5±0.1d);The group B(19.1±0.3d)and the group C(19.4±0.1d); The group B and C respectively compared with A P<0.05;But no difference was found between group B and C, P>0.05.The average gestational age the group A earlier group B about 1.5 days.(2)Total of Cx43(Total-Cx43)protein in smooth muscle tissue of the three groups were expressed, the group A of protein expression (0.81±0.03)was significantly higher than B group (0.47±0.06), increased compared with group B (34±6)%, P<0.05;Also significantly higher than the C group (0.46±0.06), increased compared with group C 34±5)%, P<0.05;There were no significant differences of the group B and C, P>0.05.(3)Phosphorylated of Cx43 (P-Cx43)protein in smooth muscle tissue of the three groups were expressed, the group A of protein expression (0.72±0.11)was significantly higher than B group (0.35±0.07), increased compared with group B (38±15)%,P<0.05;Also significantly higher than the C group (0.36±0.09), increased compared with group C(36±7)%, P<0.05;There were no significant differences of the group B and C, P>0.05.Conclusions Uterine smooth muscle P-Cx43 expression associated with premature launch; reduce the expression of Cx43 phosphorylation may inhibit uterine smooth muscle contraction; progesterone on the expression of Cx43 phosphorylation may be the prevention and control down one of the mechanisms of preterm delivery.