The Preparation Of Advanced Bioactive Glass And The Research Of It In Wound Healing In Diabetic Rats

Presently the methods of preparation of bioactive glass have extended from the traditional melting method to the sol-gel method, hydrothermal method, template method and so on. In these methods we can obtain bioactive glass powder, mesoporous bioactive glass , bioactive glass fiber and other advanced bioactive glasses, which have some better biological properties, such as the chemical homogeneity in molecular level, nanoscale mesoporous structure, larger surface area, higher chemical activity and adsorption characteristics. The bioactive glass(SiO245%, Na2O 4.5%, CaO 24.5%, P2O5 6%(45S5)) prepared using the traditional melting method has been widely used in wound healing, but only a few research of the advanced bioactive glass has been done in this area The subject of the study is is to prepare the advanced bioactive glass by the sol-gel and template methods and explore its application and mechanism in woundhealing.Nanoscale bioactive glass (NBG) powders were prepared using the sol-gel co-precipitation and freeze-drying methods in this study, also effects of polyethylene glycol (PEG-10000) as a dispersing agent on their nanoscale morphology and bioactivity was investigated. The results showed that NBG particles prepared without without adding PEG were found with an irregular particle morphology and the particle size was less than 50 nm .With addition of PEG, the spherical bioactive glass particles formed and the particle size ranged in approximately 40～100 nm. The higher PEG concentration was, the particle size decreased.The in vitro bioactivity test in a simulated body fluid (SBF) demonstrated that NBG possessed a higher bioactivity than the sol-gel derived bioactive glass(SGBG).Three kinds of bioactive glassSGBG,NBG and 45S5 were used to heal the wound in dibabetic rats. The results indicated that:○1 The bioactive glass can promote wound healing of diabetic and increase the healing rate.○2 Compared with the 45S5, SGBG and NBG can promote wound healing of diabetic rats more quickly and efficiently ,and the wound healing time is less, but no significant difference was observed between SGBG and NBG.