| Objective:Irritable bowel syndrome (IBS) is a common clinical disease with disorder of the gastrointestinal functions, and it may be associated with psychological factors. The clinical symptoms of IBS include abdominal pain, abdominal distension and abnormal stool which occurs persistently or intermittently. The incidence of IBS is 20 to 50 percent in gastrointestinal clinic. The usually treatment is symptomatic treatment. At present, there is no radical means.IBS is a kind of psychosomatic diseases with a high incidence and a long course. Its pathological mechanisms are complex. IBS belongs to functional disorder diseases/disorders. Therefore, it can be successfully accomplished by the administration of chinese herbs. Our experiment focuses on diarrhea-predominant irritable bowel syndrome (IBS-D). The aim of our study is to investigate the influence of chinese herbs on the treatment of IBS-D in model rats.Methods:1. Animal model of compound diarrhea was induced by a lactose enriched diet in the Wistar rat, combining with restraint stress. The traditional method for restraint stress and drenched with senna infusion were combined to develop another model of compound diarrhea.2. Groups with different concentrations of experimental herbs were established. Serum D-xylose content, the activity of LDH, MDH and lactase in intestinal organizations were inspected in rats. The action and mechanism of experimental herbs in intestinal digestion and absorption were explored in order to determine the best concentration of experimental herbs.3. The waves of the jejunum smooth muscle's movement in rats were recorded by the biological signal acquiring and processing system. The impact of the experimental herbs for intestinal motility was probed by testing the frequency and amplitude of the waves.4. Lactulose (L) and mannitol (M) were force-fed to the rats. The amounts of L and M as well as the ratio of L/M in urine were detected by high performance liquid chromatography (HPLC) to show the permeability of intestinal mucosa.5. The AQP4 concentration in proximal colon was detected by enzyme linked immunosorbent assay (ELISA). Results:1. The compound diarrhea animal model was induced triumphantly by Wistar rat. Diarrhea and weight loss had happened. Pathological injury in small intestinal could be found by the general microscope.2. In the light microscope, the intestinal mucosa injury decreased in medicated group.3. The activity of LDH and MDH in intestinal organizations increased remarkably in the group of medium doses. But serum D-xylose content and the activity of lactase seem no change.4. Medium doses decreased the amplitude of the jejunum smooth muscle's movement remarkably. No significant effect on the frequency of the movement.5. The ratio of L/M in urine decreased significantly in medium doses group.6. Chinese herbs had no effect on the AQP4 concentration in medicated groups and control group.Conclusion:Lactose enriched diet combining with restraint stress model rats and drenched with senna infusion combining with restraint stress model rats can induce the clinical symptoms of IBS-D. Through increasing the activity of LDH and MDH, the experimental herbs adjust the sugar's catabolism, improve the intestinal absorb, promote the liberation of energy. The amplitude of the movement of the jejunum smooth muscle had been decreased, the experimental herbs mitigate the convulsion of the muscle; adjust the intestine function of transmission, increase the absorption of the liquid in the intestine. The permeability of intestinal mucosa had been reduced; the experimental herbs are of potential action on protection to permeability of intestinal mucosa and enhancement of intestinal barrier. The results show that the experimental herbs have the regulation effect on IBS-D.
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