The Expression Of RECK,MMP-2,TIMP-2 And P21ras In Laryngeal Carcinoma And The Clinical Significance

Objective: The carcinoma of larynx is one of the malignant neoplasm in otolaryngology and head and neck surgery. The occurrence of laryngeal cancer, as other malignant tumors, is caused by the change of various gene expression levels. Reversion-inducing-cystein- rich protein with kazalmotifs (RECK) gene is the recent discovery of the transcriptional suppressor gene.RECK can inhibit tumor invasion and metastasis by inhibiting the expression of a variety of MMPs. Matrix metallo proteinases (MMPs) plays an important role in degrading extracellular matrix (ECM). Some studies have shown that the expression levels of MMP2 is closely related to the malignant degree and invasion of tumor. TIMP-2 as an inhibitor of MMP-2, inhibit the activity of MMP-2 through a variety of ways. At the same time RECK is regulated by a variety of oncogene. In our study,our aim is to investigate the correlation between the expression of RECK, MMP-2, TIMP-2, P21ras and the development of the cancer of larynx.Methods:1 Immunohistochemistry for RECK, MMP-2, TIMP-2, P21ras expression was performed on cryostat section from 40 laryngeal carcinoma ,15 para-carcinoma and 15 normal laryngeal mucosa specimens.2 Flow Cytometrye(FCM) for RECK, MMP-2, TIMP-2,expression was performed from 40 laryngeal carcinoma ,15 para-carcinoma and 15 normal laryngeal mucosa specimens.3 SPSS 13.0 was applied to analyze the results of experiment.P≤0.05 indicating significant difference.P≤0.01 indicating very significant difference.Results:1 The expression of RECK protein 1.1 The results detected by IHC : The expression of RECK ancensus from laryngocarcinoma to para-carcinoma and normal laryngeal mucosa tissues , there is statistic significance(P<0.01). The expression of RECK in laryngocarcinoma is relatively weaker than those in para-carcinoma(P<0.01) and in normal laryngeal mucosa(P<0.01)tissues respectively, we found no significant difference between the expression of para- carcinoma and normal laryngeal mucosa tissues(P>0.05). The expression of RECK was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05), but that wasn't correlated with patients'clinical classification, tumor size, smoking history, patients'age and sex(P>0.05).1.2 The results detected by FCM : The quantitative expression of RECK protein(FI=0.8682±0.1961) in laryngeal carcinoma tissues is relatively weaker than those in para- carcinoma(FI=0.9889±0.1785,P<0.01)and in normal laryngeal mucosa(FI=1.1200±0.1153,P<0.01)tissues respectively. There is no significant difference was found between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of RECK was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05).2 The expression of MMP-2 protein2.1 The results detected by IHC : The expression of MMP-2 descends from laryngocarcinoma to para-carcinoma and normal laryngeal mucosa tissues , there is statistic significance(P<0.01). The expression of MMP-2 in laryngocarcinoma is relatively higher than those in para-carcinoma(P<0.01) and in normal laryngeal mucosa(P<0.01)tissues respectively. No significant difference was found between the expression of para-carcinoma and normal laryngeal mucosa tissues(P>0.05). The expression of MMP-2 was correlated with lymph node metastasis, clinical stage and pathological grading of tumor(P<0.05), but that wasn't correlated with patients'clinical classification, tumor size, smoking history, patients'age and sex(P>0.05).2.2 The results detected by FCM : The quantitative expression of MMP-2 7 protein (FI=2.6088±0.1954) in laryngeal carcinoma tissues is relatively higher than those in para-carcinoma (FI=1.0322±0.1577,P<0.01) and in normal laryngeal mucosa (FI=0.6811±0.1732,P<0.01) tissues respectively. We found no significant difference between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). The expression of MMP-2 was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05).3 The expression of TIMP-2 protein3.1 The results detected by IHC : The expression of TIMP-2 descends from laryngocarcinoma to para-carcinoma and normal laryngeal mucosa tissues , there is statistic significance(P<0.01). The expression of TIMP-2 in laryngocarcinoma is relatively higher than those in para-carcinoma(P<0.01) and in normal laryngeal mucosa(P<0.01)tissues respectively.There is no significant difference between the expression of para- carcinoma and normal laryngeal mucosa tissues(P>0.05). The expression of TIMP-2 was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.01), but that wasn't correlated with patients'clinical classification, tumor size, smoking history, patients'age and sex(P>0.05).3.2 The results detected by FCM : the quantitative expression of TIMP-2 protein(FI=3.6077±0.1966) in laryngeal carcinoma tissues is relatively higher than those in para-carcinoma(FI=1.1125±0.1672,P<0.01)and in normal laryngeal mucosa(FI=0.6080±0.1987,P<0.01)tissues respectively, no significant difference was found between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). The expression of TIMP-2 was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.01).4 The expression of P21ras protein : The expression of P21ras descends from laryngocarcinoma to para-carcinoma and normal laryngeal mucosa tissues , there is statistic significance(P<0.01). The expression of P21ras in laryngocarcinoma is relatively hihger than those in para-carcinoma(P<0.01) 8 and in normal laryngeal mucosa(P<0.01)tissues respectively.We found no significant difference between the expression of para- carcinoma and normal laryngeal mucosa tissues(P>0.05). The expression of P21ras was correlated with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05), but that wasn't correlated with patients'clinical classification, tumor size, smoking history, patients'age and sex(P>0.05).5 The correlation of RECK and MMP-2, TIMP-2, P21ras in laryngeal carcinoma tissues5.1 The results detected by FCM: There is a negative correlation between the quantitative expression of RECK protein in laryngeal carcinoma tissues and the expression of MMP-2(r=0.895,P=0.000).The regression equation is Y=-2.206X+4.076.5.2 The results detected by IHC: There is no correlation between the quantitative expression of TIMP-2 protein in laryngeal carcinoma tissues and the expression of RECK(r=-0.208,P=0.198>0.05).5.3 The results detected by IHC: There is a negative correlation between the expression of RECK protein in laryngeal carcinoma tissues and the expression of P21ras.(r=-0.332,P=0.036<0.05)Conclusions:1 Both RECK, MMP-2, TIMP-2, P21ras protein express in all laryngeal carcinoma, para-carcinoma mucosa and normal mucosa tissues.2 The qualitative expression of MMP-2, TIMP-2 and P21ras protein in laryngeal carcinoma tissues are relatively higher than those in para-carcinoma and in normal laryngeal mucosa tissues respectively. The qualitative expression of RECK protein in laryngeal carcinoma tissues are relatively weaker than those in para-carcinoma and in normal laryngeal mucosa tissues respectively. It may contribute to the carcinogenesis and development of laryngeal carcinoma.3 The expression of RECK, MMP-2, TIMP-2, P21ras protein are correlated with malignant biological behavior of laryngeal squamous cell carcinoma, which included lymph node metastasis, patients'clinical classification and pathological grading of the tumor. The expression of RECK, MMP-2, TIMP-2, P21ras aren't related to tumor size, smoking history, patients'age and sex.4 There is a negative correlation between the quantitative expression of RECK and MMP-2. There is no correlation between the quantitative expression of RECK and TIMP-2. There is a negative correlation between the expression of RECK and P21ras.