The Expression And Significance Of Reck, HER-2/neu And VEGF In Laryngeal Carcinoma

Objective: The carcinoma of larynx is one of the malignant neoplasms in otolaryngology and head and neck surgery. As other kinds of carcinomas, the etiology and development of laryngeal carcinoma are concerned with the changed related genetic expression which is caused by multiple factors. RECK (reversion-inducing-cysteine-rich protein with Kazal motifs) is a novel discovered inhibiter of matrix metalloproteinases (MMPs) and tumour suppressor gene. It can inhibit the expression of MMPs at post-transcriptional levels. The key action of RECK is to inhibit carcinomas'invasion, development, metastasis and angiogenesis. HER-2/neu is a carcinoma gene which encodes the second human epidermal growth factor receptor. It regulates the growth and differentiation of cells and plays a demonstrated role in cell signal transduction. It induces down-regulation of RECK, resulting in the promotion of growth and invasion of tumor cells. VEGF (vascular endothelial growth factor) is an important regulation factor of inducing angiogenesis. RECK has the significant influence both on tumor angiogenesis and the biological action of tumor cells. In this study, the expression and significance of RECK, HER-2/neu and VEGF in laryngeal carcinoma are investigated and then their relationships with the genesis and development of the carcinoma of larynx are explored.Methods:1 Immunohistochemistry for RECK, HER-2/neu and VEGF expression was performed on cryostat sections from 40 laryngeal carcinoma, 30 para-carcinoma and 14 normal laryngeal mucosa specimens.2 Flow Cytometrye (FCM) was used to quantify the concentrations of RECK, HER-2/neu and VEGF in 40 laryngeal carcinoma, 30 para-carcinoma and 14 normal laryngeal mucosa specimens. 3 SPSS 13.0 was applied to analyze the experiment results. P≤0.05 indicates significant difference; P≤0.01 indicates very significant difference.Results:1 The expression of RECK1.1 The results detected by IHC: There was statistic significant difference (P<0.01) among expression of RECK from laryngocarcinoma, para-carcinoma and normal laryngeal mucosa tissues. The expression of RECK in laryngocarcinoma was relatively weaker than those in para-carcinoma (P<0.01) and in normal laryngeal mucosa tissues (P<0.01). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of RECK was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05); but it was not related with patients'clinical classification, tumor size, smoking history, patients'age and sex (P>0.05).1.2 The results detected by FCM: the quantified expression of RECK (FI=0.8682±0.2935) in laryngeal carcinoma tissues was relatively weaker than those in para-carcinoma (1.0472±0.1756, P<0.01) and in normal laryngeal mucosa tissues (1.1550±0.1714, P<0.01). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of RECK was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05).2 The expression of HER-2/neu2.1 The results detected by IHC: There was statistic significant difference (P<0.01) among expression of HER-2/neu from laryngocarcinoma, para-carcinoma and normal laryngeal mucosa tissues. The expression of HER-2/neu in laryngocarcinoma was relatively higher than those in para-carcinoma (P<0.01) and in normal laryngeal mucosa tissues (P<0.01). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of HER-2/neu was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05); but it was not related with patients'clinical classification, tumor size, smoking history, patients'age and sex (P>0.05).2.2 The results detected by FCM: the quantified expression of HER-2/neu (FI=1.5492±0.3812) in laryngeal carcinoma tissues was relatively higher than those in para-carcinoma (1.1213±0.2432, P<0.01) and in normal laryngeal mucosa tissues (1.0379±0.1760, P<0.01). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of HER-2/neu was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05).3 The expression of VEGF3.1 The results detected by IHC: There was statistic significant difference (P<0.01) among expression of VEGF from laryngocarcinoma, para-carcinoma and normal laryngeal mucosa tissues. The expression of VEGF in laryngocarcinoma is relatively higher than those in para-carcinoma (P<0.01) and in normal laryngeal mucosa tissues (P<0.05). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of VEGF was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05); but it was not related with patients'clinical classification, tumor size, smoking history, patients'age and sex (P>0.05).3.2 The results detected by FCM: the quantified expression of VEGF (FI=2.1973±0.3240) in laryngeal carcinoma tissues was relatively higher than those in para-carcinoma (1.4310±0.2923, P<0.01) and in normal laryngeal mucosa tissues (1.2729±0.1519, P<0.05). No significant difference was observed between the expression of para-carcinoma and normal laryngeal mucosa tissues (P>0.05). Expression of VEGF was related with lymph node metastasis, clinical stage and pathological grading of tumor (P<0.05).4 There is a negative linear correlation between the quantitative expression of RECK protein and HER-2/neu protein in laryngeal carcinoma tissues (r=-0.752, P=0.000). The regression equation is Y=2.053?0.581X.5 There is a negative linear correlation between the quantitative expression of RECK protein and VEGF protein in laryngeal carcinoma tissues (r=-0.805, P=0.000). The regression equation is Y=2.656?0.528X.6 There is a positive linear correlation between the quantitative expression of HER-2/neu protein and VEGF protein in laryngeal carcinoma tissues (r=0.610, P=0.000). The regression equation is Y=1.301+0.563X.Conclusions:1 RECK, HER-2/neu and VEGF express in laryngeal carcinoma, para-carcinoma mucosa and normal laryngeal mucosa tissues.2 The quantitative and qualitative expression of RECK protein in laryngeal carcinoma tissues are relatively weaker than those in para-carcinoma and normal laryngeal mucosa tissues. It may contribute to the carcinogenesis and development of laryngeal carcinoma.3 The quantitative and qualitative expressions of HER-2/neu and VEGF in laryngeal carcinoma tissues are relatively higher than those in para-carcinoma and normal laryngeal mucosa tissues. They may contribute to the carcinogenesis and development of laryngeal carcinoma.4 The expression of RECK, HER-2/neu and VEGF are related with malignant biological behavior of laryngeal squamous cell carcinoma, such as lymph node metastasis, patients'clinical classification and pathological grading of the tumor. The expression of RECK, HER-2/neu and VEGF are not related with patients'clinical classification, tumor size, smoking history, patients'age and sex.5 There is a negative linear correlation between the quantitative expression of RECK and HER-2/neu, VEGF.6 There is a positive linear correlation between the quantitative expression of HER-2/neu and VEGF.