Study On The Influences Of Scalp-point Penetration Needling On Endogenous Neural Stem Cell Proliferation And Differentiation Of Acute Cerebral Ischemia Rats

Objective:Cerebrovascular disease is high in morbidity, mutilation rates and case fatality, and ischemic cerebrovascular disease is the most common one. A great deal of clinical researches and empirical studies show that scalp-point penetration needling has unique curative effect on this disease. Cerebral infarction lead to patients'loss of nerve function and neuron death. Currently, focus of the study with cerebral infarction is how to actively and effectively contribute to recovery neurological deficits caused by stroke. We made rat focal cerebral ischemia model with middle cerebral artery occlusion, using a thread block technique. We gave a mark of the neurologic impairment in rats. In this way, we are trying to explore the mechanism of scalp-point penetration needling in cerebral ischemia from endogenous neural stem cell proliferation and differentiation.Methods:64 SD rats were selected. Each rat's weight was from 200g to 250g. They were randomly divided into four groups, each group with 16 rats:normal group, sham group, cerebral ischemia group and scalp-point penetration needling group. Normal group rats were not provided treatment. Scalp-point penetration needling group were made focal cerebral ischemia model with middle cerebral artery occlusion, using a thread block technique. It was treated by EA on Baihui(GV20) through Qianding(GV21) and Shuaigu(GB8) through Xuanli(GB6) after the rats woke up. The treatment continued for seven days, one vice for 30 minutes per day. Then, we marked of the neurologic impairment in the rats and immunohistochemical detection of hippocampal deneate gyrus (HDG) Nestin. The expression of GFAP mRNA and NSE mRNA in the hippocampus was assayed by method of real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results:1. Focal cerebral ischemia rats, with right middle cerebral artery occlusion, using a thread block technique had behavior disorder to some degree, with levo-circling objective signs, and their left forefoot flection couldn't expand thoroughly. This showed that the CI models succeeded. After treated by scalp-point penetration needling, neurologic defect objective signs of rat improved obviously. Compared with cerebral ischemia group, there was significant difference (P<0.01).2. Compared with normal group and sham operated group, the expression of Nestin in rats'hippocampal dentate gyrus in scalp-point penetration needling group increased greatly (P<0.01). Compared with cerebral ischemia group, the expression of Nestin in rats'hippocampal dentate gyrus significantly increased (P<0.01).3. Compared with normal group and sham operated group, the expression of GFAP mRNA in the hippocampus was increased, in scalp-point penetration needling group increased greatly (P<0.01). Compared with cerebral ischemia group, the expression of GFAP mRNA in the hippocampus was decreased significantly (P<0.01).4. Compared with normal group and sham operated group, the expression of NSE mRNA in the hippocampus was decreased in scalp-point penetration needling group increased greatly (P<0.01). Compared with cerebral ischemia group, the expression of NSE mRNA in the hippocampus was increased significantly (P<0.01).Conclusion:1. After acute cerebral ischemia, the expression of Nestin in rats'hippocampal dentate gyrus increased, and the expression of GFAP mRNA in the hippocampus was increased, and the expression of NSE mRNA in the hippocampus was decreased. It shows that Nestin, GFAP mRNA and NSE mRNA took the pathologic diagnosis of cerebral ischemia.2. The neurologic impairment of rats with crerbral ischemia improved obviously by scalp-point penetration needling, indicating that scalp-point penetration needling can repair cerebral ischemia damage.3. The expression of Nestin in rats'hippocampal dentate gyrus was increased by scalp-point penetration needling, and decreased the expression of GFAP mRNA, and increased the expression of NSE mRNA. It shows that the method of scalp-point penetration needling may promote endogenous neural stem cell proliferation and differentiation in acute cerebral ischemia rats.