Expressions Of CXCL12/CXCR4 And CXCL16/CXCR6 In Cervical Carcinoma

Objective To observe the protein expressions of chemokines CXCL16, CXCL12 and their exclusive receptors CXCR6, CXCR4 in cervical carcinoma, and to detect their significance in the invasion of cervical carcinoma and migration of lymphocytes.Methods Specimens of cervical carcinoma, CIN, and normal cervical tissue were collected and processed into tissue chips. The protein expressions of chemokines CXCL16, CXCL12 and their exclusive receptors CXCR6, CXCR4 in the samples were analyzed by immunohistochemistry. The relationship between the expression of chemokines CXCL12 and its receptor CXCR4 in cervical carcinoma and FIGO stage, metastasis of lymph nodes, the degree of differentiation, the type of pathology, and the age of patient were analyzed. Using SPSS11.5, the protein expressions of chemokines CXCL16, CXCL12 and their receptors CXCR6, CXCR4 were analyzed by X2-test, and the relationship between CXCL12/CXCL16 and CXCR4/CXCR6 were analyzed by Spearman test. The significant size of test of this study was validated as value of 0.05.Results Chemokines CXCL16, CXCL12 and the receptors CXCR6, CXCR4 have high-level expressions in cervical carcinoma (83.33%,78.33%,83.33%,80%), which showed significant difference compared with CIN and normal cervical tissue (P< 0.05). The expression of CXCL12 was closely related to lymph node metastasis (P<0.05). Oppositely, it was not associated with FIGO stage, the degree of differentiation, the type of pathology, and the age of patient (P>0.05). The expression of CXCR4 protein was closely related to lymph node metastasis and FIGO stage (P<0.05), but it was not associated with the degree of differentiation, the type of pathology, and the age of patient (P>0.05). The expressions of chemokines CXCL16, CXCL12 was positively correlated with their receptors CXCR6, CXCR4 in cervical carcinoma(r=0.436 and 0.517, P<0.01).Conclusion The high-level co-expression of CXCL12/CXCR4 and CXCL16/CXCR6 implied that the interactions between CXCL12 and CXCR4, CXCL16 and CXCR6 play important roles in the progression and lymph node migration of human cervical carcinoma.