The Study Of Clinical And Experimental Immunology With Yiqi Yangyin Huoxue In SLE

Systemic lupus erythematosus (SLE) is a violation of multiple systems and organs of a typical autoimmune disease. Glucocorticoid,the treatment of this disease,which is one of the most effective drugs in modern medice, but the long-term use of corticosteroids may lead to many side effects, while in the process of glucocorticoid Withdrawal,the disease often occur repeatedly. In recent years, researches have shown that Chinese medicine treatment can reduce the amount of glucocorticoid and overcome the side effects,so they make play a significant role, which is subjected to the attention of many physicians.Objective:This study on SLE patients with Lang No.1 (Yiqi Yangyin Huoxue Principle is belong to Bian Tianyu, the well-known expert in Integrative Medicine skin) before and after treatment to observe the relative level of gene transcription factor Foxp3 and cytokine IL-10, TGF-β. and changes in their clinical research. So as to analyze the elucidating pathogenetic of SLE and to analyze the pharmacological mechanisms of the immune with Yiqi Yangyin Huoxue Principle, which lay the foundation for further development and utilization of Lang No.1, which can be a breakthrough of progress in treating incurable diseases such as SLE.Methods:The expression of transcription factors Foxp3 in PMBC from patients with SLE before and after treatment with Lang NO.1 and the control group before and after treatment and healthy controls was detected by fluorescent real-time quantitative PCR, H, the levels of IL-10 and TGF-βin serum from patients with Lang NO.1 and the control group before and after treatment and healthy controls was detected by ELISA; and analyze laboratory indicators and clinical symptoms are improved or not.Results:1.RT-PCR detection of Foxp3 expression in SLE lower than the control group, the difference was statistically significant;2. ELISA test results show that IL-10 in SLE group was higher than the normal control group, TGF-βlower than the control, the difference was statistically significant.3. The treatment group and control group before and after treatment and found that Foxp3 expression with Lang 1 treatment was higher than before treatment, IL-10 was lower than those before treatment, TGF-βhigher than before treatment, the difference was significant; the control group has not significant changes before and after treatment, which was not statistically significant.4. In clinical studies, the treatment group on SLE in clinical symptoms, laboratory indicators were better than the control group; integrated effect was higher than the control group; and can reduce the amount of hormones so as to reduce the complications and adverse reactions.Conclusion:1. RT-PCR detection of Foxp3 expression in SLE lower than the control group, the difference was statistically significant. We can speculate that Foxp3 in the pathogenesis of SLE have a certain sense, we suggest that regulatory T cells in the pathogenesis of SLE makes an important role.2.ELISA test results show that IL-10 SLE group was higher than the normal control group, TGF-βlower than the control, the difference was statistically significant. We can speculate that Tr function with SLE patients of IL-10 secretion increased, while reducing the secretion of TGF-β.3. From before and after treatment,both clinical indicators and immune changes, the full explanation of Chinese medicine in the treatment of SLE has played a pivotal role, and to avoid or mitigate the side effects of hormone therapy,and to alleviate the suffering of patients, which was reflecting the treatment of traditional Chinese medicine with multiple links,multi-target site and multi-channel features.