Studies On Processing Principles Of Euphorbia Ebracteolata Hayata

The processing principle research for Euphorbia ebiacteolata Hayata.was one of the topics named as "study of Processing Techniques and Principles of toxic drugs" of the State Admini-stration of Traditional Chinese Medicine, which discussed the processing principle of langdu, mainly through the aspects of chemistry, pharmacology, etc., to provide the theoretical basis for making the quality standard of the processed sample. Main research contents:1.Literature researchThrough the study on medical literatures about Euphorbia processing from past dynasties and the standards of herbal processing practiced in different districts in China, the author summed the history of Euphorbia processing, and its original meaning and gave suggestions to improve the Euphorbia processing and lay a theoretical foundation for the processing principle of langdu.2.Chemical researchWe assay the extraction liquid for the raw and processed samples of langdu with HPLC un-der different absorption wavelengths.The result of the contrasted altas analysis manifested that changes occurring to the content.The content of 2,4-dihydroxy-6-methoxy-3-methyl-acetophenone; 2-hydroxy-6-methoxy-3-methyl-acetophenone-4-β-glueoside and Polysaccharide were deter-mined before and after langdu was processing. Making use of the GC-MS to compare the chemical composition in the expressed oil before and after processed of euphorbia.The chemical compounds of the processed langdu were systemically separated, 2,4-dihydroxy-6-methoxyl-methylene-3-methyl-acetophenone,2,4-dihydroxy-6-methoxyl-methyl ene-3-methyl-acetophenone-4-O-β-D-glucopyranoside,octacosyllate,3-acetyl-β-amyrin,3,3'-diac etyl-4,4'-dimethoxy-2,2',6,6'-tetrahydroxy diphenylmethane,2,4-dihydroxy-6-methoxy-3-methyl acetophenone, gallic acid,2-hydroxy-6-methoxy-3-methyl-aeetophenone-4-β-glueoside, P-sitosterol and daucosterol;ferulic acid,4-hydroxybenzoic acid,β-stigmasterol, hexadecanoic acid were respectively received from the petroleum and ethyl acetate extract of langdu.3.Pharmacological researchA method was established to compare activities of acute toxicity, anti-inflammatory, anti-oxidant and MTT method was developed to compare the activity of antitumor in vitro of dief-frent raw and processed samples of langdu,which also provided the scientific basis for clarifying the processing principle of langdu.