Experiment Study On Treating The Mesangial Proliferative Glomerulonephritis With Umbellate Pore Decoction Combined Herbal Of Strengthening Spleen & Qi

Objective:The intention of this topic is to make the model of MsPGN immunization. The MsPGN rats were cured by Umbellate Pore Decoction Combined Herbal of Streng-thening Spleen&Qi (Astragalus, Codonopsis, Atractylodes) 24h urine protein, urine occult blood, Scr and BUN, the expression of renal a-SMA and TGF-β1, pathological changes, were observed. Further verify the efficacy of the treatment MsPGN with Umbellate Pore Decoction Combined Herbal of Strengthening Spleen&Qi. And its mechanism of action was explored in cellular and molecular level. For the Umbellate Pore Decoction Combined Herbal of Streng-thening Spleen&Qi provide a better experimental basis for clinical application.Therapys:A total of 40 Wistar rats of 7 weeks old were divided into four groups randomly, male and female half and half:blank control group (GroupⅠ), pathological model group (GroupⅡ),Umbellate Pore Decoction group(GroupⅢ), Umbellate Pore Decoction Combined Herbal of Strengthening Spleen&Qi group(GroupⅣ). The rats model of MsPGN was established as the Zhang Wuxing's improved model of chronic serum sickness nephritis, and was treated with the appropriate drug. After four weeks treatment, the general situation in rats, the 24h urine protein, urine occult blood, Scr and BUN were observed. The change of Kidney organization pathological was observed with conventional light microscope. The expression ofα-SMA and TGF-β1 were observed with the immunohistochemical staining. The results of immunohistochemistry were analyzed with the pathologyimage analysis system.Result:(1)24h urinary protein quantitative and Urine occult blood analysis showed that: Compared with the blank control group, pathological model group 24-hour urine protein and Urine occult blood was significantly higher (P<0.01); GroupⅢand GroupⅣ24-hour urine protein and urinary occult blood compared with the pathological model group decreased significantly (P<0.01), and the two compared in 24 hour urine protein and the urine occult blood both have statistics difference (P<0.05). (2) Renal function analysis showed that: BUN and Scr in pathological model group were higher than blank control group (P<0.01); Meanwhile, Scr and BUN in GroupⅢand GroupⅣdescented significantly than blank control group (P<0.01). And the two compared in Scrand BUN both have statistics difference (P<0.05). (4) In nephridial tissue, immunohistochemistry result ofα-SMA and TGF-β1: Compared with blank control group, the expression ofα-SMA and TGF-β1 was enhanced obviously in pathological model group (P<0.01). Compared with pathological model group, the expression ofα-SMA and TGF-β1 was diminution obviously in GroupⅢand GroupⅣ(P<0.01), an two group compared has statistics difference (P<0.05). (5) Nephridial tissue pathomorphology showed that:In model group, the mesangial region expanded obviously, matrix increased, index of mesangial matrix increased, prompted to copy the successful model; Compared with the model group, the above pathology change of GroupⅢand GroupⅣobviously reduced, mesangial cell proliferation reduced, matrix index remarkable dropped, and GroupⅣis changes light compared with GroupⅢ.Conclusions:Through this experimental study discovery that:(1)Umbellate Pore Decoction Combined Herbal of Strengthening Spleen& Qi can reduce 24h urine protein, urine occult blood, Scr, BUN effectively, reduce the renal histopathological damage and protect renal function of the MsPGN rats. (2) Umbellate Pore Decoction Combined Herbal of Strengthening Spleen&Qi can effectively inhibit the mesangial cells proliferation of MsPGN rats, reduce the accumulation of mesangial matrix, delay MsPGN pathological progress. Its mechanism may be related to be able to reduce the renal glomerularα-SMA and TGF-β1 expression. (3) Umbellate Pore Decoction Combined Herbal of Strengthening Spleen&Qi can be effectively treated MsPGN, And its overall efficacy is better than simply using Umbellate Pore Decoction, seems suggest MsPGN at the same time with the syndrome of Yin-deficiency and water-heat combined, also with the potential pathogenesis of Qi deficiency of Spleen, its provided a new way for clinical treatment MsPGN.