Expression And Significance Of RECK And MMP-2 In Patients With Gestational Trophoblastic Disease

Objective:To investigate the expression of Reversion-inducing-cysteine-rich protein with kazal motifs and Matrix metalloproteinase-2 in normal villus and hydatidiform moles and lately malignant transformed hydatidiform moles,and try to explore the role of them in the malignant transformation of hydatidiform moles.Method:The expression of RECK and MMP-2 were detected by immunohistochemistry in 20 cases of normal human villus, and 24 cases of benign moles,24 cases of malignant moles.Results:1.The expression of RECK in gestational trophoblastic disease.RECK was chiefly located in cytoplasm and cell membrane.The expression of RECK ascend gradually from malignant mole group, benign mole group to normal villus group.The positive expression rate of normal villus group was 90%,79.2% in hydatidiform mole group,58.3% in malignant moles group.There was a significant difference between normal villus group and malignant mole group.2.The expression of MMP-2 in gestational trophoblastic disease.MMP-2 located in cell cytoplasm and cell membrane. There was obvious difference of MMP-2 positive rate among normal villus group,benign mole group and malignant mole group. The positive expression rate of normal villus group was 35%,and 83.33% of hydatidiform mole,100% of malignant moles,which had significant difference between normal villus group and benign mole group.There is also a statistically significance between benign mole group and malignant mole group.3.There is a negative correlation between RECK and MMP-2 expression by correlativity analysis..4.The rate of MMP-2 being negative expression with RECK being negative expression in benign mole was higner that in malignant mole, which has statistically significance. Conclusion:The disbalance between RECK and MMP-2 take part in the malignance transformation of hydatidiform mole.lt has important value of MMP-2/RECK in discriminating benign mole and malignant mole.