Studies On The Quality Criterion Of Flowers Of Eiobotrya.japonica (Thunb) Lindl.

Loquat flower is the dried flowers of Eriobotrya japonica (Thund) Lindl. which belongs to rosaceae family. This species mainly distributed in Sichuan, Hunan, Hubei, Zhejiang, Fujian, Chongqing and other places in China.Loquat flower has been used for curing cold cough, snuffle and runny nose, cough with hemoptysis, head stroke and so on. It is usually used to treat Feire cough in children and chronic cough in folk. Flower thinning carries every year to present the highest quality fruit, which about 60~80% fresh flowers will be picked and then discard them like garbage. This is a terrible waste of plant resources. Our reaserch group focuses on the basic research of loquat flower to explore its medicinal value. So it is necessary to draw up the quality criterion of flowers of Eiobotrya.japonica (Thunb) Lindl.There are seldom reports about the researeh of loquat flower. 13 different batch of loquat flowers were collected from main distribution regions in China for researching the quality criterion, which taken Chinese Pharmacopoeia ChP (2005 edition) as the basic references. The main research contents will be reported shortly as follows.In this thesis, research has done on microscopical chatacteristics and TLC identification method of loquat flower. The content of total flavonoids and total triterpenoids were determined with ultraviolet spectrophotometry. A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the determination of oleanolic acid and ursolic acid at one time. The fingerprints of loquat flower was established by using HPLC with the peak of ursolic acid as reference. The research supplemented the deficiency of study of water content, ash and extractives content. Residues of organochlorine pesticides in loquat flower were determined by solid-phase extraction and capillary gas chromatography, while organophosphrous and pyrethroid pesticides residues were determined by gas chromatography-mass spectrometry. We refered to the latest edition of Chinese Pharmacopoeia ChP (2010 edition) when writting the quality criterion of flowers of Eiobotrya.japonica (Thunb) Lindl.The research result of this thesis provided a feasible criterion to controle quality of loquat flower and also supplied a gap of quality criterion about loquat flower.