| Phellinus Igniarius (Polyporaceae, Basidiomycotin) is a rare leechdom fungus. It was one kind of Chinese Tradition Medicines to treat many diseases like dysenteria, night-sweat, uterine bleeding and as emictoria in Japan. Researchs respected that it had a good effect on cancer, fibrosis and can enhance i-immunity. It contains active components of triterpene, polysaccharide, flavone, and it is a rare fungus which contains flavone compounds had the effect of anti-OX, broaden blood vessel and antitumous.The articles mainly studys the extraction, depuration, pharmacodynamic action and praeparatum technology for exploitation and utilization, the main reaserach contents have been listed as followings:1. The extraction rate of total flavonoids as the indictor, compare the five different species of Phellinus Igniarius, the contents of total flavonoids listed from higher to lower was Phellinus yucatanensis, Phellinus vaninii with Betula platyphylla Suk., Phellinus igniarius with Betula dahurica Pall., Phellinus igniarius, Phellinus pini.2. Optimum conditions for extracting flavone from Phellinus vaninii sporophore by ultrasound-assisted method were studied using monofactorial and orthogonal testing. It was concluded that the optimal conditions were:particle size 40-60 mesh, alcohol ratio 70%, extraction temperature 45℃, ratio of liquid to solid by 30:1, extraction time 20 minutes, power 50W.3. For studying on Antioxidation effects of total flavonoids from Phellinus vaninii by different purification methods, experiments on Fe3+ disoxidation, reducing power, Fe2+ complexation, DPPH clearance were adopt, with comparising with crude flavonoids and polysaccharide from Phellinus vaninii. Result displays that the effect of crude flavonoids is the best but the content of flavonoids had raised that purified through macroreticular resins chromatography, polyamide chromatography, n-butanol extraction.4. Senium mouse models administered D-GAL by hypodermic injection was raised with alcoh extract and aqueous extract from Phellinus vaninii by intragastric administration respectively, after 15 days detected NOS and NO in brain. Result displays that the both contents are cut down and the effects of dosage parts have no difference. Hyperlipemia mouse models were administered higher, middle, lower dose total flavonoids from Phellinus vaninii by lavage, they were killed after 15 days and were and detected organ quotient, MDA,SOD of blood-serum and No in brain, result displays that total flavonoids from Phellinus vaninii have the role in lowering blood-fat.5. The disintigrate as the index, monofactorial and orthogonal experimental tests were used to optimizate dispersible tablet preparation technology of total flavonoids from Phellinus vaninii, the best was MCC:LYP(6:2)as loading agent,5%PVP K17 in alcoh as binder,7% super-CMS as disintegrating agent, pressure 3kg.6. To establish a method of ultraviolet spectrophotometry after charge-transfer, aluminum chloride was selected as the acceptor; the contents of total flavonoids in extract of Phellinus vaninii were assayed. The result:A=0.028C-0.001, R2=0.9994, linear within range 1～40μg·ml-1, average recovery 99.59%.7. RP-HPLC method for determination of naringenin in flavone dispersible tablet of Phellinus vaninii was set up. YWG-C18 column (4.6mm×250mm,5μm) was used. The detection wavelength was set at 288nm. The mobile phase consisted of methanol-water-aceticacid (40:59:1) with the flow rate 0.6 ml·min-1 and column temperature at 40℃. The standard curve A=9.499×105C+698.8 (R2=0.9994), linear within range 1～100μg·ml-1. The method RSD was RSD 1.1%(n=6), the average recovery of assay was 103.3%(RSD=1.5%, n=9), the RSD of reproducibility experimentation was 0.3%(n=6) and the test was stable in 10 hours. The results:the content of naringenin in flavone dispersible tablet of Phellinus vaninii was 0.0343% (RSD=0.7%,n=6)...
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