Experimental Research And Forensic Meaning Of Diagnosing Anaphylactic Shock Using Tryptase And IgE

Objective:Anaphylactic shock is a tough problem for both forensic experts and clinic doctors. We studied IgE immunochemical staining of digestive tract and respiratory tract. The serum levels of IgE and tryptase were also tested using radio-immunity technique. We wish to find sensitive methods for forensic identification of anaphylactic shock.Methods:18 guinea pigs are classified into two groups which are the testing group and the control group with no purpose (Every group contains 9 guinea pigs). Establishing guinea pigs anaphylactic shock model, and making specimens of blood serum, digestive tract and respiratory tract, we tested the serum levels of IgE and tryptase with radio-immunity method, and we also stained IgE using immunohistochemistry. Results:Compared with the control group,(1) The serum levels of IgE and tryptase rise(p＜0.05). (2) IgE immunochemical staining shows that positive expressions are higher than that of control group. Conclusion: Compared with the control group, when anaphylactic shock happen, (1) The serum levels of IgE and tryptase rise. (2) Expression of IgE become strong. Therefore, IgE immunochemical staining and radio-immunity examination of IgE and tryptase, may be useful to diagnose anaphylactic shock.