An Immunohistochemical Study On The Expression Of Substance P In Human Sudden Erethistic Death

Objective To study the expression of Substance P in human sudden erethistic death, and to show mast cell degranulation by using combine –staining methods,trying to seek objective morphological reference supports to diagnose sudden erethistic death for forensic medicine. Methods (1)The expression of SP were performed on the 15 human lung,laryngopharynx and gastrointestinal tissue of anaphylactic shock and 20 human coronary heart disease as control by immunohistochemical technique. And the images of SP are analyzed by BI-2000 image analyzer, and the positive indexes (PI) are calculated. (2)Using BB-IG-MY combine-staining methods of mast cell. Results (1)SP expression is significantly stronger of the laryngopharynx and gastrointestinal tissue in the experimental groups than in the control ones (p<0.001). Statistic t test result is: laryngopharynx mucosa group t=6.942, laryngopharynx gland group t=4.601, gastrointestinal tissue group t=5.025. (2) The expression of SP around the bronchia has no immunohistochemical-staining result in both experimental groups and control ones. (3) Mast cell is showing clearly by the combine-staining methods, and the cytoplasmic granule is deep brown, the cell nuclear is green and red blood cell is yellow; And mast cell nondegranulation is in the control groups, while mast cell degranulation is in the experimental ones. Conclusion SP expression was significantly stronger in the laryngopharynx and gastrointestinal tissue of sudden erethistic death, and mast cell degranulation is clearly shown by combine-staining methods. So they can offer an objective morphological reference support for diagnosing sudden erethistic death in forensic medicine.