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The Effects Of Different Exercise Intensity On Myosin Heavy-chain (MHC) Isoforms Gene Expression In Quadriceps Femoris Muscles Of Male Rats During Growing

Posted on:2004-11-01Degree:MasterType:Thesis
Country:ChinaCandidate:C Y XuFull Text:PDF
GTID:2167360095453714Subject:Human Movement Science
Abstract/Summary:PDF Full Text Request
The purpose of this study is to investigate the effects of different exercise intensity on myosin heavy chain (MHC) isoforms mRNA expression in skeletal muscles of male Spraque -Dawley(SD) rats during growing . 100 SD male rats that is only four weeks old were randomly divided into the base contrast group, low load exercise group and high load exercise group. In low and high load exercise group were again divided into 3 week group,6 week group and 9 week group. At the same time in order to avoid the reciprocity of normal growth with exercise, the contrast groups were especially set up relevantly. In each group there were ten rats. They were fed in different chests taking food and drinking freely. The exercise were carried out 3 weeks 6 weeks and 9 week. The rats of the exercises groups carried out continuous uphill running with the speed of 12m/min, 20m/min for 60 min (degree + 5 ). The base contrast group were slay before the day which the other groups started. 3 W 6 W and 9 W groups were slay 24 hours after exercise. A Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) method is used to measure the quantities of MHC isoforms mRNA of quadriceps femoral muscle.The findings from these studies can be summarized as follows: 1) There was no change in the MHC mRNA expression of the sedentary group. 2) Low load exercise or high load exercise exercises have no effects on the quantities of MHC-I mRNA in quadriceps femoris muscles of male rats during growing, and from this we can conclude that exercise training can't affect the expression level of MHC-I gene of muscle. 3) The content of MHC-IIb mRNA isoform decrease and the content of MHC-I MHC-IIx mRNA isoform raises after low load exercise or high load exercise. But the effect of low load exercise on MHC-Ila mRNA expression in quadriceps femoris muscles of male rats during growing is greater than that of high load exercise. And the effect of high load exercise on MHC-IIx mRNA expression in quadriceps femoris muscles of male rats during growing is greater than that of low load exercise. 4) The more longer exercise time that was carried, the more notable effect of exercise on MHC-II mRNA expression in quadriceps femoris muscles of male rats during growing.
Keywords/Search Tags:exercise, myosin heavy-chain (MHC), gene expression, growth
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