The Research Of Sports Training On Rats' Spatial Cognitive Learning And Memory And On The Ultrastructure Of Their Hippocampus And Immunohistochemistry

Many documents show that exercises of different intensity exert different influence on central nervous system. In particular, sports of long length or fatigue training result in the micro damage of brain structure and function, and will cause the change of the elements, which influence learning and memory. This thesis deals with the neurobiology of mouse memory under the exercises of different intensity and also studies the microstructure and immunohistochemistry of hippocampus.There are all together 34 male SD rats, which are provided by the Medical School of Xi'an Jiaotong University. They are feed naturally by standard dry feed, their weights are 248?4g and they live under the temperature of 22C-27C, and momisture of 40% -60%. After one week of natural feeding, they are randomly divided into three groups: sedentary group (11 rats), aerobic training group (1 lrats) and fatigue training group (12 rats). Aerobic training group and fatigue training group received 3-day training, with the speed of 15m/min in 15min. Aerobic training group: 5 days per week, 8 weeks. Accelerating speed of 3m/min in 5min to 20m/min. After60min, to add 5 to the treadmill. Fatigue training group: 6 days per week, 8 weeks, by using brush and wood bar to stimulate mice. Accelerating speed of 3m/min in 5min to 20m/min. After60min, to add 10 to the treadmill. After 8 weeks of training, Morris water maze is adopted to examine the memory ability of the rats. Then the relevant material of rats will be observed by applying electron microscope and immunohistochemistry observation. The changes of the ultrastructure of CA1 and CA3 areas of rats' hippocampus are observed by transmission electron microscope. The changes of nNOS, iNOS, eNOS, VEGF, NSE, NF-k Bp65, Bax and Bcl-2 are observed by applying immunohistochemistry SABC technique.The experiment shows:1) Exercises of different intensity can restrain the increasing of the rats' weights, especially for the fatigue training group.2) Exercises of different intensity do not influence the learning ability and spatial cognitive ability of the rats, and the fatigue training influences their memory ability.3) Aerobic training causes the changes of neuron of CA1 and CA3 areas, glial cell, blood capillary and synapse microstructure. Fatigue training can cause damage the ultrastructure of neuron of CA1 and CA3 areas, glial cell, myelinated nerve fiber, and synapse mircostructure.4) Fatigue training intensifies nNOS expression in CA1 CA3 areas of hippocampus, especially for aerobic training. In Purkinje cell, aerobic training intensifies nNOS expression, while fatigue training restrain nNOS expression.5) Fatigue training intensifies iNOS expression in CA1 CA3 areas of hippocampus, especially for aerobic training. In Purkinje cell, fatigue training restrains iNOS expression.6) Fatigue training restrains eNOS expression in CA1 area of hippocampus, especially for the aorobic training group. Fatigue training restrains eNOS expression in CA3 of hippocampus, and aerobic training obviously restrains eNOS expression in Purkinje cell.7) Fatigue training promotes VEGF expression in CA1 area of hippocampus, especially for the aerobic training. Aerobic and fatigue training restrain the VEGF expression of CA1 area of hippocampus, while the aerobic training promotes the VEGF expression in Purkinje cell.8) Aerobic training and fatigue training restrain NSE expression in CA1 area of hippocampus. As for its expression in CA3 area, aerobic training promotes it, and fatigue training promotes it more obviously. However, aerobic and fatigue training restrain NSE expression.9) Aerobic training and fatigue training promote NF- k Bp65 expression in CA1 area of hippocampus. Aerobic training restrains NF- k Bp65 expression in CA3 of hippocampus. But in Purkinje cell, aerobic training restrains NF- k Bp65 expression, while fatigue training promotes NF- k Bp65 expression.10) Aerobic training restrains Bax expression in CA1 area of hippocampus, especially for aerobic training. Aerobic training...