| Associated with aging occurs, the body cardiovascular system gradually aging. The size of myocardial cells reduced some old people, apoptosis increased changes in such patterns as a result of myocardial degeneration, resulting in blood supply to the heart function can not fulfill the normal physiological. Age-related loss of muscle mass (sarcopenia) is one of the most marked symptom associated with aging. It's consequences mainly reflected in loss of muscle mass,muscle size and muscle strength.In accordance with the concept of skeletal muscle sarcopenia, the concept of myocardial sarcopenia was proposed Myocardial sarcopenia is that cardiac myocyte dysfunction and cardiovascular reduced with age. Regular exercise can produce adaptation to myocardial hypertrophy, and as a result of exercise-induced cardiac hypertrophy. And pathological cardiac hypertrophy in the structure and function of the nature of the differences, which, through campaigns to improve as a result of aging caused by myocardial contraction provides a theoretical basis.there was a significant change in the process of cardiac hypertrophy of MAPK family ERK and JNK and the proto-oncogene family of c-Fos and c-Jun.And the two families there may be some correlation, MAPK family may be based on the form of signaling molecules through the family of proto-oncogene activation mechanism of signal transmission mechanism of cardiac hypertrophy.This study of elderly male SAMP8 mice and treadmill training ladder to observe the age and the two training SAMP8 mouse model of myocardial function of the form, as well as ERK, JNK, c-Fos and c-Jun mRNA level of expression of to explore the aging process in the presence of myocardial contraction, as well as through the ladder and treadmill training for these two modes of intervention in the aging process is to produce cardiac hypertrophy or delayed myocardial contraction. Experimental Methods: 40 subjects for SAMP8 mice were divided into young control group (YC), aging control group (OC), age group endurance (OE) and the aging resistance training group (OR). Using the treadmill and ladder weight as resistance training and endurance training in the training mode. Application of real-time quantitative PCR detection method to determine the ERK, JNK, c-Fos, and c-Jun mRNA expression variation.Experimental results: 1) After 8 weeks of training, the mice weight had no significant change, and the age factor on the mice have a greater impact on the heart, the heart of aging mice HI of the control group significantly less than the control group of young mice hearts HI, P <0.05; in elderly mice in two models through the heart of the training coefficients are significantly higher, P <0.05, but between two modes of training had no significant changes in heart HI.2) Intervention through the campaign, two different training modes ERK expression increased in mouse cardiac aging than the aging control group significantly, P <0.05; but it had no significant changes between aging endurance group resistance training group.3) Aging endurance group JNK mRNA expression increased than the aging control group, P <0.05; resistance training group than the aging control group there is an upward trend, comparison between the two training patterns, the result was no significant difference.4) training in two different mouse model, the c-Fos mRNA expression increased in both,P<0.01;the group of endurance training is the more obvious increase than the resistance training group,P<0.05.5) compare with the training in two different modes of aging mice and the control group, the myocardial expression of c-Jun mRNA were increased, P <0.01; resistance training group increased more significantly than endurance training group, P<0.05.Conclusion: 1) two forms of exercise training on elderly delayed myocardial contraction, resulting in adaptation have an active role in hypertrophy, heart coefficient increased significantly. However, the two training models was no significant difference between the passage of this experiment can not determine which is more conducive to training older people to postpone cardiac atrophy.2) exercise training, heart of ERK, JNK, c-Fos and c-Jun mRNA expression significantly increased on exercise-induced cardiac hypertrophy and ERK, JNK, c-Fos and c-Jun are closely related.3) MAPK family and the proto-oncogene family, both in the exercise-induced cardiac hypertrophy process are closely related, MAPK family, ERK, JNK may be a member of the family as the proto-oncogene c-Fos, c-Jun of the emerging role of signal molecules.
|
PDF Full Text Request