Experimental Study On Intervention Of Brain Aβ In Coronary Atherosclerotic Small Pigs By Invigorating Spleen And Removing Phlegm And Removing Blood

The purpose:This experiment by observing the spleen and eliminating phlegm blood method for coronary atherosclerosis miniature pig blood lipid and brain tissue LXR- beta, the influence of ABCA1, Apo E, A beta, discusses the spleen expectoranting blood circulation method of atherosclerosis model miniature pig brain A beta intervention effect. Further discusses the spleen expectoranting blood party action mechanism of prevention and treatment of alzheimer’s disease, provide scientific experiment basis for clinical prevention and treatment of alzheimer’s disease.The experimental materials and methods:55 6-8 months of age only male guangxi miniature pig according to the weight in 2 groups at random: normal group 5, scores50. Normal group was given basic diet, high scores to heat a high-fat feeding, a moderate amount of bile salt rijin appetite for last week says 3% of the weight, free drinking water,adaptive feeding 1 week. In 2 weeks for coronary balloon extrusion with strain(8 only in the process of balloon extrusion die, 42 survived only), 42 miniature pig to survive by random number table method, a new stochastic grouping, scores miniature pigs can be divided into:the model group(n = 7), spleen group(n = 7), spleen, eliminating phlegm group(n = 7),eliminating phlegm blood group(n = 7), spleen, eliminating phlegm and blood group(n = 7),western medicine group(n = 7). Each miniature pigs before in accordance with the feed at the same time, the corresponding treatment of traditional Chinese medicine compound mixing in the diet, model group and normal group was given the same volume of pure water, feeding to24 weeks, 24 after operation are based on the weekend. The miniature pig and fixed on the operating table, anesthesia, open the abdominal cavity, abdominal aortic blood, death, gather the brain. This experiment chose only normal group(n = 5), model group(n = 7), spleen,eliminating phlegm group(n = 7), expectoranting blood group(n = 7), spleen, eliminating phlegm blood group(n = 7), automatic biochemical analyzer were used to detect the concentration of TC, TG, HDL, LDL and immunohistochemical method to detect brain tissue LXR- beta, the expression of ABCA1, Apo E, A beta. All results of this experiment measured using SPSS17.0 statistical analysis, the results with the average + /- standard deviation(- X +S) said, compares the single factor analysis of variance between groups. Statistically significant(P < 0.05).Results:1. Compared with normal group, model group Obama miniature pig of TC, TG, HDL, LDL concentration significantly increased(P < 0.01), spleen and eliminating phlegm and eliminating phlegm group, spleen, eliminating phlegm blood group compared with model group, TC, TG, HDL and LDL levels were significantly lower(P < 0.01), with statistical significance(P < 0.05). Spleen and eliminating phlegm and eliminating phlegm group, spleen,eliminating phlegm blood group of lipid effect each have differences,(P < 0.01), spleen expectoranting group to adjust the TC, HDL and LDL ability stronger than the expectorant blood and spleen expectoranting blood group, with statistical significance(P < 0.05); Spleen and eliminating phlegm blood group TG regulating ability stronger than the spleen and eliminating phlegm and eliminating phlegm blood group, with statistical significance(P <0.05).2. Compared with normal group, model group of miniature pig brain LXR- beta, the expression of ABCA1 and Apo E were significantly increased(P < 0.01); Spleen and eliminating phlegm and eliminating phlegm group, spleen, eliminating phlegm blood group compared with model group, brain tissue LXR- beta, the expression of ABCA1 and Apo E were significantly increased(P < 0.01); Spleen and eliminating phlegm blood group of the spleen and eliminating phlegm and eliminating phlegm group effect, with statistical significance(P < 0.05); Spleen and eliminating phlegm and expectorant effect, though there are differences between blood group, but no statistical significance(P > 0.05).3. Compared with normal group, model group of miniature pig brain A beta significantly higher(P < 0.01); Spleen and eliminating phlegm and eliminating phlegm group, spleen,eliminating phlegm blood group compared with model group, brain A beta decreased significantly(P < 0.01); Spleen and eliminating dampness and phlegm group effect is better than that of the spleen and eliminating phlegm and eliminating phlegm group, with statistical significance(P < 0.05); Spleen and eliminating phlegm and expectorant effect, though there are differences between blood group, but no statistical significance(P > 0.05).Conclusion:1. The spleen and eliminating phlegm blood can adjust the coronary atherosclerosis model Obama miniature pig blood lipid level.2. Lipid abnormalities can promote atherosclerosis model Obama miniature pig brain A beta.3. The spleen and eliminating phlegm blood may be through improving LXR- beta, the expression of ABCA1, Apo E, reduce A beta deposition in the brain, in order to reduce the expression of A beta.