| Purpose:Through between acupoint injection of bone marrow mesenchymal stem cells and cardiac muscle injection of bone marrow mesenchymal stem cell treatment for acute myocardial infarction in rats, and compared the two injection transplantation method for the improvement of heart function and observed between the two kinds of bone marrow mesenchymal stem cell transplantation means of vascular cell adhesion factor 1(VCAM 1), late-onset antigen 4(VLA-4), 1 alpha matrix derived factor(SDF-1), 1 alpha receptors(CXCR4) matrix derived factors such as the expression of cytokines. To find out a by applying the method of cell transplantation in treatment of acute myocardial infarction(MI) of new and effective treatment measures.Material and method:This experiment chooses the animals to clean SD rats and two grade clean male SD rats. Clean level of SD rats weight is: 100 g + 10 g, it is mainly used for this experiment of cell culture; Clean level male SD rats weight: 250 g + 10 g, mainly used in the experiment of animal building. First grade clean only 60 male SD rats were randomized, divided into: control group, model group, the myocardial stem cell injection group and the acupuncture point injection of stem cells, each group of 15 only(control refers to the mice after thoracotomy, to the left anterior descending coronary artery thread only, without ligation. Its significance lies in the exclusion of animals in experiments of reaction is caused by the possibility of building).After the completion of the group, through the use of the anterior descending coronary artery ligation to copy the model of acute myocardial infarction, specific methods are as follows: the distance between the left edge of the rib 3 rats sternum to 0.5 cm, with scissors longitudinal do an organization is about 1.5 cm long, incision and vascular clamp intercostals muscles and its surrounding all scattered small blood vessels in the clip, and use little, forceps in 3 ribs on both sides of the rib gap through double ligature, along both sides of the ligature blunt separation intercostals muscles, and at the two ends of the 3rd rib ligation intercostals arteries, again from both sides ligature central cut 3 ribs and gently pull the vision fully exposed to the right, and then open the dirty pleura and pericardium, to separate the thymus tissue, from the left atrium and pulmonary artery cone find and myocardial surface between courses and main line to the heart of the left anterior descending coronary artery(LAD) and application specifications for 720 noninvasive suture needle in left heart below the ears of about 2 mm and a noninvasive suture, and through the heart muscle layer outline, beside the pulmonary arterial cone out fine needle. Then observe ecg changes, for its stability returns after 15 minutes, ligation in heart surface area a is about 0.5 cm long, 1.5 cm wide soft cotton in the blood vessels in slipknot ligation of left anterior descending coronary artery(the control group underwent ligation thread but not only), ecg monitoring and shown when observed Ⅱlead ST segment is a carpenter raise up, and show that when the heart corresponding to a dark area on the surface of the acute myocardial infarction(mi) mode of operation is completed.Different dosage groups, the myocardial stem cell transplantation groups: when the rats of acute myocardial infarction(mi) building success for 72 hours, immediately after the second open thoracic surgery under direct on myocardial necrosis areas, select six points 1 x 1010 trace syringe and apply evenly to 1.2 ml/l of stem cell cultures of transplantation in myocardium, as observed in the corresponding myocardial regional uplift is dome sample, explain stem cell transplant success. Acupoint injection of stem cell groups: when acute myocardial infarction rats 72 hours immediately after building success for the double side heart shenshu point, to Yang acupuncture point, acupuncture point injection of stem cells within four points in the shan culture 0.3 ml(1 x 1010 / l), acupoint transplantation is completed. Between bone marrow mesenchymal stem cell transplantation for 4 weeks after the start observation rat cardiac function change, cytokines, VLA(VCAM- 1-4, alpha SDF-1 and CXCR4) expression and the safety of stem cell transplantation. Among them, the heart function change main observation indexes include: brain natriuretic peptide(BNP) and left ventricular end-diastolic volume(LVEDV), end of left ventricular systolic volume(LVESV), left ventricular short axis shortening rate(FS) and left ventricular ejection fraction(LVEF). The expression of cytokines main observation indexes include: vascular cell adhesion factor 1(VCAM 1), late-onset antigen 4(VLA- 4), 1 alpha matrix derived factors(SDF- 1 alpha), matrix derived factor alpha 1 receptor(CXCR4). Transplant safety index mainly observed the rats’ blood routine, liver function, renal function and other corresponding indexes, and indirectly reflect the between bone marrow mesenchymal stem cells after security problems.Results:1.From the results of the determination of cardiac function change, acupoint injection of stem cells and myocardial injected stem cells group also showed the cardiac function has improved trend, two groups compared with model group the results showed that plasma BNP level, left ventricular end-diastolic volume(LVEDV), left ventricular end systolic volume(LVESV), left ventricular short axis shortening rate(LVFS) and left ventricular ejection fraction(LVEF), and other indicators are improved, and had statistical significance(P<0.05); And myocardial stem cell injection group and the comparison between acupoint injection stem cell group, although have numerical difference, but no statistical significance(P>0.05).2.Changes from the result of the expression of related cytokines, acupoint injection of stem cells and myocardial injected stem cells group showed a cytokine expression ability increased in the two groups compared with model group the results showed that blood vessel cells adhesion factor 1(VCAM-1), late-onset antigen 4(VLA-4), 1 alpha matrix derived factor(SDF-1 alpha), matrix derived factor alpha 1 receptor(CXCR4), and other indicators are increased, and had statistical significance(P<0.05); Myocardial injections of stem cells and comparison between groups with point injection of stem cells, although have numerical difference, but no statistical significance(P>0.05).3.From the perspective of the observation results of safety indicators, stem cells and cardiac muscle injection point injection group compared to model group of blood routine, liver function, renal function and other indicators are no significant change, no statistical significance(P>0.05); Stem cell group and the acupuncture point injection of stem cells and myocardial injection group comparison between no statistical significance(P>0.05).Conclusion:1.Confirmed by experimental data, the acupuncture point injection of stem cells and cardiac muscle injections of stem cells in both the cardiac function of acute myocardial infarction rats.2.Confirmed by experimental data, the acupuncture point injection of stem cells and cardiac muscle injections of stem cells in rats with acute myocardial infarction, vascular cell adhesion factor 1(VCAM-1), late-onset antigen 4(VLA-4), 1 alpha matrix derived factor(SDF-1 alpha), matrix derived factor alpha 1 receptor(CXCR4) four kinds of the expression of cytokines have enhanced role.3.Confirmed by experimental data, the acupuncture point injection of stem cells and the treatment of myocardial injections of stem cells from the influence of routine blood, liver, kidney function perspective all have certain security. |
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