Study On The Preparation And Physical And Chemical Control Of Stasis Extinction Temperature-sensitive Situ Swelled Gel

Stasis extinction Temperature-sensitive situ swelled gel, which is made from Radix rhubarb and Bloom honeysuckle and Radix gardenia and so on,is an effective prescription in treating acute injury of soft.The therapeutic effect is to promote blood circulation and stop pain,to Heat detumescence,to Scatter stasis dredging.We have made a systematic study its preparation and physical and chemical control,the study has laid the foundation for providing the basis.1.Study of the prescription(1)Study of the extractionOptimization of extraction technology for Temperature-sensitive situ gel, Rheum officinale and Honeysuckle flower and Fructus Gardeniae were respectively being the ethanol refluxing,decoction, dregs of a decoction using the ethanol refluxing after CO2 fluid extraction(SFE-CO2) technology, Emodin, chlorogenic acid and geniposide content and Condensate rate in the extact is index,We deal the data with the normalization and combination score method,the optimum alcohol extraction confirmed by he contradistinction investigations and the orthogonal test was:0.5 hours of soaking,2 times of refluxing with 70% alcohol,10.8 times of alcohol-adding amount and 2.0,1.5 hours of refluxing time respectively.(2)Study of the concentrationWe choose the decompression concentretion to deal with the concentrated solution with contradistinction experiment.The physic liquor was decompression concentrated at 70℃,the vacuity was-0.08 Mpa. (3)Study of the preparation processGelation temperature, gel strength and bioadhesive force of Temperature-sensitive situ swelled gel is index, optimization of Matrix matching for Stasis extinction Temperature-sensitive situ gel. Poloxamer407,Poloxamer188,Carbopol of Matrix matching for Stasis extinction Temperature-sen sitive situ gel respective is 22%,3%,0.7 %.Determined by HPLC method through skin test in drug emodin, chlorogenic acid, the accumulation of geniposide through quantity as evaluation indexes. Adopt comprehensive pointrating method of data processing To determine the optimal transdermal absorption promoting agents was 6% nitrogen ketone.2.Study of Physical and chemical control indexrheum officinale, radix angelicae,Mentha haplocalyx and honeysuckle flower were identified respectively by TLC.The content of Emodin, Chlorogenic acid and Geniposide were determined by HPLC.A new high performance liquid chromatography method was developed;the average recovery rate of Emodin was 99.25%,while RSD was 1.11%, the average recovery rate of Chlorogenic acid was 99.28%,while RSD was 1.09%,and the average recovery rate of Geniposide was 98.77%,while RSD was 1.75%.The method was reliable,precise and can be applied for Physical and chemical control.3. Preliminary pharmacodynamics studyStasis extinction situ swelled gel and the original dosage orm of nitrogen respectively by 6% for transdermal testosterone promoter. Determined by HPLC method through skin t est in drug emodin, chlorogenic acid, the accumulation of geniposide through quantity as evaluation indexes.Stasis extinction situ swelled gel,48 h elimination of the original for the cumulative permeation amount of gel were emodin 25.6μg·cm-2, chlorogenic acid 133.8μg·cm-2, Geniposide 227.3μg·cm-2. Cumulative permeation amount of he original formulation compared to bruising, eliminate the accumulation of in situ gel permeation more. Stasis extinction situ swelled gel with the original formulation of consumer preparation of high and ow concentrations, respectively, determined by plate method against Staphylococcus aureus inhibition zone diameter, bruising, eliminate situ gel zone of inhibition of Staphylococcus aureus Larger than the original formulation.